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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255, R155A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD52

Plasmid

#172456

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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mKO-Fibrillarin-7

Plasmid

#57842

Purpose

Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Fibrillarin (FBL Human)

Use

Tags

mKO

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS1964

Plasmid

#82574

Purpose

High copy number plasmid containing Ple305 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple305

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pIRES-EGFP-TPD52

Plasmid

#172460

Purpose

Expression of untagged Tumor Protein D52 (TPD52) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPG_2

Plasmid

#86345

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1806

Plasmid

#154319

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1805

Plasmid

#154318

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::3xMyc for CT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1348

Plasmid

#154311

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker for NT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1822

Plasmid

#154322

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Promoter

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
AL08_pgRNA.DMD

Plasmid

#100293

Purpose

DMD intron 43-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6 Polymerase III snRNA

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KLF9

Plasmid

#86286

Purpose

Donor vector for 3' FLAG tag of human KLF9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF9 homology arms (KLF9 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_2

Plasmid

#86347

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_1

Plasmid

#86346

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD1_2

Plasmid

#104043

Purpose

Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD1 gRNA (GATAD1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD1_1

Plasmid

#104042

Purpose

Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD1 gRNA (GATAD1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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