We narrowed to 3,043 results for: NAK

1. pPBpuro-cRaf-mNG-eDHFR(69K6)

   Plasmid

   #172107

   Purpose

   Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   cRaf-mNeonGreen-eDHFR(69K6) (RAF1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIN-Gpi1

   Plasmid

   #175934

   Purpose

   knockout mouse Gpi1

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Gpi1 gRNA (Gpi1 Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335-sgRNA-EF(Cas9n)-mHdac1-L2

   Plasmid

   #122307

   Purpose

   Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Hdac1 (Hdac1 Synthetic)
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL Neo Myr Flag TBK1

   Plasmid

   #20648

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_TBK1_WT

   Plasmid

   #82285

   Purpose

   Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   N388D; K570Q

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

   Plasmid

   #127659

   Purpose

   Over-express EGFP-NLS-FLAG-cGAS

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Tags

   EGFP-NLS-Flag

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

   Plasmid

   #134925

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

   Plasmid

   #134924

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTubb3-MC

    Plasmid

    #87112

    Purpose

    minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFP
    

    Use

    CRISPR

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-RAD52-WT

    Plasmid

    #235676

    Purpose

    Expresses HA-tagged wild type human RAD52 in mammalian cells.

    Depositor

    Simon Powell

    Article

    Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

    Insert

    RAD52 (RAD52 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 10xHis-3C-BRD4(42-459) BD1-BD2

    Plasmid

    #233886

    Purpose

    pRSF-DUET1 for protein overexpression of human BRD4

    Depositor

    Alessio Ciulli

    Article

    Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    10x his 3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pClneoMyc-LATS1

    Plasmid

    #66851

    Purpose

    Express Myc-tagged LATS1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    LATS1 (LATS1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P44L mutation in LATS1 compared to GenBank refere…

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2_mSTING_gRNA_1

    Plasmid

    #196625

    Purpose

    Knock-out STING in murine cells, gRNA 1.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 1 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPRv2_mSTING_gRNA_2

    Plasmid

    #196626

    Purpose

    Knock-out STING in murine cells, gRNA 2.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING gRNA 2 (Sting1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXN2-HA-AT1R-YFP

    Plasmid

    #101659

    Purpose

    Expresses AT1R with HA tag and YFP in mammalian cells.

    Depositor

    Yusuke Ohba

    Article

    Inuzuka et al Sci Rep. 2016 Feb 9;6:21613. doi: 10.103

    Insert

    Angiotensin II type 1 receptor (AGTR1 Human)
    

    Tags

    HA and YFP (Venus)

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS

    Plasmid

    #127656

    Purpose

    Over-express NLS-FLAG-cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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