We narrowed to 3,254 results for: actin

1. tetO-HAND2

   Plasmid

   #170690

   Purpose

   doxycycline-inducible overexpression of HAND2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   HAND2 (HAND2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CIB1-mCerulean-MP

   Plasmid

   #58366

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-EGFP.Flag-EZH2-mt2

   Plasmid

   #220245

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-CIB1-mCherry-Rab8A

   Plasmid

   #194333

   Purpose

   For use in light-induced Rab8a protein inactivation through the interaction with CRY2

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Inserts

   CIB1 (CIB1 Mustard Weed)
   Rab8a (RAB8A Canis Lupus)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-HNF1A-WT-Flag-UTR

   Plasmid

   #183237

   Purpose

   Expression of FLAG tagged HNF1A

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Insert

   HNF1 homeobox A (HNF1A Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2 LRP6-APEX2-Ires-eGFP-PAC

   Plasmid

   #180142

   Purpose

   used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ad:2CMV_A2R2-N25K3

   Plasmid

   #122242

   Purpose

   Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

   Depositor

   Corey Neu

   Article

   Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

   Inserts

   C-terminal end of Nesprin-3 (Syne3 Mouse)
   Actinin alpha 2 (Actn2 Mouse)
   

   Use

   Adenoviral

   Tags

   Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-CIB1-mRFP1-MP

   Plasmid

   #58367

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Tags

   mRFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cntn1-Fc-His

   Plasmid

   #72065

   Purpose

   Expresses the extracellular region of the Contactin 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Cntn1 (Cntn1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p(HA)HIF1alpha(401delta603)R27G

    Plasmid

    #52215

    Purpose

    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

    Depositor

    Eric Huang

    Article

    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

    Insert

    HIF1alpha (401delta603) R27G (HIF1A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 401-603 deleted, removes the oxygen-dependent …

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pMRX-IP-muGFP-hATG2A ΔWIR

    Plasmid

    #215507

    Purpose

    Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    Autophagy related 2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    muGFP

    Expression

    Mammalian

    Mutation

    P656R (natural variant with no functional relevan…

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cntn2-Fc-His

    Plasmid

    #72066

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cntn2-AP-His

    Plasmid

    #71940

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cntn5.1-AP-His

    Plasmid

    #71943

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cntn5.1-Fc-His

    Plasmid

    #72069

    Purpose

    Expresses the extracellular region of the Contactin 5, isoform 1 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn5.1 (Cntn5 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)

    Plasmid

    #239353

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    MALAT1 ENE(WT)-mascRNA(mut 8356-8370)
    

    Expression

    Mammalian

    Mutation

    MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…

    Available Since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR-B_2xsgRNAs_RPP21 (pAVA3259)

    Plasmid

    #239327

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RPP21

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RPP21 (RPP21 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPR-B_2xsgRNAs_BRF2 (pAVA3258)

    Plasmid

    #239326

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting BRF2

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting BRF2 (BRF2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. TCTN3 minigene (pAVA3939)

    Plasmid

    #239345

    Purpose

    Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)
    

    Tags

    3xFLAG, 6xMyc, and CopGFP

    Expression

    Mammalian

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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