We narrowed to 3,352 results for: pEGFP
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Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyExpressionMammalianMutationAmber and Ochre stop codons in the former linker …Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-tdTomato-VPR
Plasmid#232632PurposeVPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-tdTomato-VPR
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK
Plasmid#208612PurposeMammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2clust-mCherry-RANK (Cry2 Mustard Weed, Mouse)
TagsmCherryExpressionMammalianPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-INPP5E-CAAX
Plasmid#202746Purposeexpress plasma membrane targeted fluorescent protein-tagged enzymeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert22aa-eDHFR(69K6)-EGFP
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAT9750-BEAR-mCherry
Plasmid#162990PurposeBEAR target plasmid with split mCherry and disrupted 5' splice siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
vsv-CenpB 1-166-Sgo1 N61I-GFP
Plasmid#108499Purposeexpression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCenpB-Shugoshin 1 (SGO1 Human)
TagsEGFP and VSVExpressionMammalianMutationCenpB 1-166 fusion before Sgo1, no PP2A binding N…PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP ∆CBM
Plasmid#100728PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
TagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motifPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iCoChR++
Plasmid#98173Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSynthetic construct iCoChR++ gene
TagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…PromoterCMV (+enhancer)Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFF-EGFP
Plasmid#86061PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFibrocystin (Pkhd1 Mouse)
TagsGFPExpressionMammalianMutationcontains luminal and transmembrane domains of CD8…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
SL2-sgSat-MTSb/BFP/pdCas9-C1
Plasmid#162761PurposeExpressing dCas9 and sgRNA containg MTSa targeting centromeresDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9 and sgRNA(SL2-sgSat-MTSb)
ExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1C-BioID2-HA (KIF1C Human)
TagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Double
Plasmid#100729PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
TagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motif and …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD8a-hnRNP-M-EGFP
Plasmid#86054PurposeA mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD8a (CD8A Human)
TagsGFPExpressionMammalianMutationCD8a luminal and transmembrane domians along with…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD8a-cNLS-EGFP
Plasmid#86052PurposeA mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.DepositorInsertCD8a (CD8A Human)
TagsGFPExpressionMammalianMutationCD8a luminal and transmembrane along with two SV4…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATF4 15: 5’ATF4.uORF1stopm1.GFP
Plasmid#21862DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATF4 (Atf4 Mouse)
TagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…Available sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-PH-BTKx1
Plasmid#183651PurposePtdIns(3,4,5)P3 biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBTK (BTK Human, Frog)
TagsX. laevis map2k1.L(32-44)-EGFPExpressionMammalianMutationAmino acids 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
TagsC-terminal CAAX-box and mVenusExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only