We narrowed to 3,233 results for: pEGFP

Showing: 2641 - 2660 of 3233 results

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GFP-DroshaS300/302A

Plasmid

#62530

Purpose

Serines 300 and 302 of Drosha are mutated to alanines

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serines 300 and 302 changed alanines (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serines 300 and 302 to alanines

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
ITPKA-mTurquoise2

Plasmid

#137811

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA (ITPKA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hela kinase-dead MLCK-GFP

Plasmid

#46317

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase-dead (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

E1626K

Promoter

CMV

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only
FKBP-beta2(616-951)-GFP

Plasmid

#100726

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(A)

Plasmid

#87913

Purpose

Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499A

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PH-Btk(R28C)-GFP

Plasmid

#51464

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Tags

GFP

Expression

Mammalian

Mutation

R28C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pmCherry-Zdk1-EB1C

Plasmid

#107695

Purpose

C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

Zdk1 and mCherry

Expression

Mammalian

Mutation

EB1 aa 186-268

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai1

Plasmid

#73561

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

NM_032790.3 (ORAI1 Human, Synthetic)

Tags

G-GECO1

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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Fz4-GFP

Plasmid

#42197

Depositor

Robert Lefkowitz, Jeremy Nathans

Article

Chen et al Science. 2003 Sep 5;301(5638):1391-4.

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pH2R-mCherry-N1

Plasmid

#84326

Purpose

Histamine-2 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H2R (HRH2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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SSpB-HaloTag-p63DH

Plasmid

#176116

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2clust-mCherry-RANK

Plasmid

#208612

Purpose

Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

Depositor

Tomohiro Ishii, Takao Nakata

Article

Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

Insert

CRY2clust-mCherry-RANK (Cry2 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-beta1(617-949)-GFP

Plasmid

#100732

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP1B1 (AP1B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-MCS-eDHFR(69K6)-EGFP

Plasmid

#172101

Purpose

Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

22aa-eDHFR(69K6)-EGFP

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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SSpB-mCherry-p63DH

Plasmid

#176112

Purpose

Heterodimerization with iLID, RhoA activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

RHOGEF p63 (ARHGEF25 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-GFP

Plasmid

#72893

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Plasmid

#173862

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pssGFP-hCRT

Plasmid

#80022

Purpose

Mammalian expression of signal peptide-EGFP-humanCRT

Depositor

Chinten James Lim

Article

Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

Insert

CRT (CALR Human)

Tags

EGFP and signal peptide

Expression

Mammalian

Mutation

signal peptide 17aa added before GFP-CRT, will ta…

Promoter

CMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only