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Plasmid#41664DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; C-terminal hyd…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE H-Ras 12V, 189L
Plasmid#14718DepositorInsertH-ras (HRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V, 189LPromoterAvailable sinceAug. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG/MKRN1(1-280)
Plasmid#78752PurposeTo overexpress MKRN1(1-280) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation1-280PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG/MKRN1(110-482)
Plasmid#78754PurposeTo overexpress MKRN1(110-482) in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsFLAGExpressionMammalianMutation110-482PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCRISPomyces-2BD
Plasmid#234429PurposePlasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified dCas9(D10A, H840A) with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-ELL-CAAX
Plasmid#220076Purposean extra long linker attached to a CAAX domain with an mCherry tagDepositorInsertCAAX (HRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorInsertCG1358 (HisT Fly)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC5A7
Plasmid#218539Purposehuman SLC5A7 cDNADepositorInsertSLC5A7 (SLC5A7 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC44A1
Plasmid#218538Purposehuman SLC44A1 cDNADepositorInsertSLC44A1 (SLC44A1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTH-EGFP
Plasmid#209322PurposeAAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTH
Plasmid#209319PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 43-372
Plasmid#205054PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
UseTagsExpressionBacterialMutationLacks Mitochondrial localization signal and C ter…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , F124D, residues 108-200
Plasmid#180629PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200, F124DPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , L177D, residues 108-200
Plasmid#180634PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200, L177DPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CALPAIN-7 residues 1-165, V18D
Plasmid#180612PurposeBacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.DepositorInsertCALPAIN-7
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-165, V18D mutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CALPAIN-7 residues 1-165, F98D
Plasmid#180613PurposeBacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.DepositorInsertCALPAIN-7
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-165, F98D mutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-EGFP-HRasC20
Plasmid#187252PurposeLenticiral expression of EGFP-HRasC20DepositorInsertHRasC20 (HRAS Human)
UseLentiviralTagsEGFPExpressionMutationPromoter5' LTRAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D
Plasmid#176462Purposeexpressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13DDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D N3521S
Plasmid#176463Purposeexpressing VPS13D patient mutant N3521S in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationN3521S mutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRV∆G-N*PEST-4Cre
Plasmid#174381PurposeRabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domainDepositorInsertCre recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-LexA-VP16-MiniWhite
Plasmid#165900PurposeBlasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GCaMP6f-4x128-mAGNET
Plasmid#166023PurposeExpresses GCaMP6f in CaMKII+ cells with low miRNA-128 expressionDepositorInsertGCaMP6f with 4 miR-128 binding sites
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-mRuby2-4x128-mAGNET
Plasmid#166021PurposeExpresses mRuby in CaMKII+ cells with low miRNA-128 expressionDepositorInsertmRuby2 with 4 miR-128 binding sites
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_C433S-SHC
Plasmid#139612PurposeStrepIII-HA-tagged gene expressionDepositorInsertPTPRA_C433S (PTPRA Human)
UseTagsStrepIII-HAExpressionMammalianMutationC433SPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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PTPRA_Y789F-SHC
Plasmid#139614PurposeStrepIII-HA-tagged gene expressionDepositorInsertPTPRA_Y789F (PTPRA Human)
UseTagsStrepIII-HAExpressionMammalianMutationY789FPromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y530EEI
Plasmid#140322PurposeExpresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationY530EEIPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
RET_Y981F-SHC
Plasmid#139621PurposeStrepIII-HA-tagged gene expressionDepositorInsertRET_Y981F (RET Human)
UseTagsStrepIII-HAExpressionMammalianMutationY981FPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_026
Plasmid#123838PurposeEncodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_027
Plasmid#123839PurposeEncodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::GAI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_011
Plasmid#123726PurposeEncodes HA-tagged PIF6 fused to Tet Repressor protein as a Type 3 part to be used in the MTK systemDepositorInsertTETR::PIF6::HA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_012
Plasmid#123727PurposeEncodes HA-tagged PYLcs fused to VP16 activator domain as a Type 3 part to be used in the MTK systemDepositorInsertVP16::PYLcs::HA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1474
Plasmid#82420PurposePlasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
UseTagsHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1475
Plasmid#82421PurposePlasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
UseTagsHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
719-2µ-minHIS3
Plasmid#40610DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBMN-Z-IN
Plasmid#1735PurposeThis retroviral vector expresses lacZ and has an IRES-NEO downstreamDepositorInsertLacZ-IRES-Neo
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only