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pHEN2 F8-2

Plasmid

#186033

Purpose

Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

Promoter

lac

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-Glyco-Venus-bPAC(S27A)

Plasmid

#165490

Purpose

Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

Glyco-Venus-bPAC(S27A)-Myc

Use

Cdna expression, xenopus oocyte

Tags

Myc and Venus

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPGS dnTcf-4(deltaN41)

Plasmid

#19284

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

Tcf-4 (TCF4 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Dominant negative, lacks N-terminal 41 amino acids

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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LRRK2-K1906M

Plasmid

#35946

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

LRRK2 (LRRK2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pGL3-FLIP pr5

Plasmid

#19130

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +28…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pMIR-9

Plasmid

#25037

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

miR-9 binding site (MIR9-1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-Lin28b

Plasmid

#64795

Purpose

Expresses human Lin28b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b (LIN28B Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Klf1

Plasmid

#50785

Purpose

retroviral expression of mouse Klf1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf1 (Klf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-Lin28b-P3

Plasmid

#64794

Purpose

Luciferase expression driven by Lin28b promoter P3

Depositor

Joshua Mendell

Article

Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Lin28b promoter P3 (LIN28B Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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double_sgRNA targeting e3 and e7

Plasmid

#190689

Purpose

sgRNAs targeting enhancer 3 and 7 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 3 and 7 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouseAtoh1-IRES-GFP

Plasmid

#73572

Purpose

Mammalian retroviral expression of Atoh1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pGL3-FLIP pr4

Plasmid

#19129

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

FLIP promoter (CFLAR Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

The sequence corresponds to positions -503 to +10…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pBR322 hCDK4 promoter

Plasmid

#16571

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (CDK4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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