We narrowed to 3,348 results for: N-EGFP
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Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmEGFP and mtagBFP2ExpressionMammalianPromoterCMVAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHalo2X-C1
Plasmid#251419PurposeMammalian expression vector for expressing a protein of interest with an N-terminal HaloTagDepositorTypeEmpty backboneTagsHaloTagExpressionMammalianMutationTwo silent mustations to disable the XhoI and SalIAvailable SinceMarch 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDL001_Hec1-ms_IgG_HC
Plasmid#193618PurposeHec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.DepositorInsertAnti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
TagsHis6 and signal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL002_Hec1-ms_IgG_LC
Plasmid#193619PurposeHec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.DepositorInsertAnti-Hec1 light chain (Hec1-ms_IgG_LC)
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pDL025_HA-rb_IgG_HC_15F11
Plasmid#193622PurposeHA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.DepositorInsertAnti_HA_15F11 rabbit heavy chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL026_HA-rb_IgG_LC_15F11
Plasmid#193623PurposeHA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.DepositorInsertAnti-HA_15F11 rabbit light chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
Tagssignal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-TPD54
Plasmid#172448PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP tagDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-CIBN-GFP
Plasmid#79572Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFPDepositorInsertLyn11
TagsCIBN (CRY2-binding domain) and EGFPExpressionMammalianMutationG8R (please see depositor comments below)PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPPromoter35SAvailable SinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS
Plasmid#199348PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-TEVExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only