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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pOZ-Pax7-FH-C-puro

Plasmid

#32518

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax7d (Pax7 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pEnt L1L3-MCS

Plasmid

#26798

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Available Since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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Sox17 Flox

Plasmid

#23335

Depositor

Sean Morrison

Article

Kim et al Cell. 2007 Aug 10. 130(3):470-83.

Insert

Sex determining region Y-box containing gene 17 (Sox17 Mouse)

Use

Mouse Targeting

Mutation

None

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pLU-YFP-ICP0FxE

Plasmid

#134564

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

YFP-ICP0

Tags

None

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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FancF_N_FS_v2_Donor

Plasmid

#79891

Purpose

N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

3xFLAG_Twin_Strep

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)

Plasmid

#174527

Purpose

Dual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNB774

Plasmid

#60114

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pEYFP(C1)-NIPP1

Plasmid

#44228

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

NIPP1 (PPP1R8 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
Rab5c cds in pGem-T Easy

Plasmid

#80518

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5c (rab5c Zebrafish)

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Rab5b cds in pGem-T Easy

Plasmid

#80517

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab5b (rab5b Zebrafish)

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2

Plasmid

#79256

Purpose

Expresses EGFP-tagged DmPAN2 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1/HisMax-Syx1

Plasmid

#35708

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

6x HIS and Xpress

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pME-mVenus-NS

Plasmid

#80497

Purpose

Multisite gateway entry clone for adding N-terminal fusions of mVenus

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

mVenus (no STOP)

Use

Multisite gateway middle entry clone

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Bul

Plasmid

#21119

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-143 sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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Rab7a cds in pGem-T Easy

Plasmid

#80522

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab7 (rab7a Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab7b cds in pGem-T Easy

Plasmid

#80523

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab7b (rab7b Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab11a cds in pGem-T Easy

Plasmid

#80529

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab11a (rab11a Zebrafish)

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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