4,177 results

1. tdTomato-C1

   Plasmid

   #54653

   Purpose

   Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. cytoplasmic EKAR (Cerulean-Venus)

   Plasmid

   #18679

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Tags

   monomeric Cerulean and monomeric Venus

   Expression

   Mammalian

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC034 - LwCas13a-msfGFP-2A-Blast

   Plasmid

   #91924

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCALNL-DsRed

   Plasmid

   #13769

   Purpose

   Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   DsRed2
   

   Use

   Cre/Lox; Flp/frt

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCMV Puro DEST ERKKTRClover

   Plasmid

   #59150

   Purpose

   Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMVInt

   Plasmid

   #18935

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-NC-CRISPRi (Gen3)

   Plasmid

   #73499

   Purpose

   Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEXTK-Pak1 70-117

   Plasmid

   #12217

   Depositor

   Jonathan Chernoff

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PBD (p21-binding domain), aa 70-117

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSin-EF2-Sox2-Pur

    Plasmid

    #16577

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. BIC-Gag-CAS9

    Plasmid

    #119942

    Purpose

    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

    Depositor

    Théophile Ohlmann, Philippe Mangeot, Emiliano Ricci

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GAG FMLV fused to spCAS9
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    hCMV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCGN N-Ras wt

    Plasmid

    #14723

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE PGK-GFP-IRES-LUC-W

    Plasmid

    #46793

    Purpose

    2nd generation lentiviral vector

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Inserts

    PGK
    eGFP (eGFP )
    IRES
    Luciferase
    

    Use

    Lentiviral

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-hsp27 wt FL

    Plasmid

    #17444

    Depositor

    Andrea Doseff

    Article

    Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

    Insert

    small heat shock protein27 (HSPB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6/UbC/mSlc7a1

    Plasmid

    #17224

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    Slc7a1 (Slc7a1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. RelA cFlag pcDNA3

    Plasmid

    #20012

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Insert

    RelA (Rela Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid

    #141391

    Purpose

    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-N (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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