We narrowed to 8,354 results for: 11
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Plasmid#230986PurposeExpresses GFP tagged MLF2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMLF2 (MLF2 Human)
Tags3xFLAG and GFPExpressionMammalianPromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
Tags6xHis and FLAGExpressionBacterialMutationY39TAGAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRExpressionMammalianPromoterU6Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONRPEX18Km-SceI-pheS
Plasmid#111610PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.Available sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCx43 (Gja1 Rat)
TagsEBFP2PromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)
Plasmid#52906PurposeBinary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]+Venus
UseRetroviralTagsL-IFP-F[2]ExpressionMammalianPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ7
Plasmid#51443PurposeExpresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q7 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgAMPKa1
Plasmid#138704PurposeExpresses a human AMPKa1-targeting sgRNA and Cas9DepositorAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)
Plasmid#114067PurposeT7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guidesDepositorInsertentry vector for AsCas12a crRNAs
UseIn vitro transcriptionAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mCherry-roGFP2
Plasmid#107220PurposeMammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-roGFP2
Tags4xCox8ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
sfCherry11-620W-PTPN22
Plasmid#195386PurposeMammalian expression of a human PTPN22 mutant (620W) with sfCherry(11) tagDepositorAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_H1_Gal4VP64
Plasmid#169918PurposeExpression of a synNotch receptor containing antiCD19, a human Notch1 core, and Gal4VP64.DepositorInsertantiCD19-Notch1-GL4VP64
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG
Plasmid#140018PurposePlasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertEcoTyrRS
TagsFLAGExpressionMammalianMutationY37L, D182S, F183M, L186APromoterEF1Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZac2.1 SV40-CMV-SaCas9-3xNLS
Plasmid#78601PurposeAAV vector containing SaCas9DepositorInsertSV40-CMV-SaCas9-3xNLS
UseAAV and CRISPRTagsHA tagExpressionMammalianPromoterSV40, CMV promotersAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TetO-dCas9-mD3A
Plasmid#78255PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (WT)
Plasmid#200978PurposeMammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
TagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding DomainPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9-mRuby2
Plasmid#183872PurposepX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.DepositorTypeEmpty backboneUseCRISPRTagsHypaCas9-P2A-mRuby2-T2A-PuroExpressionMammalianPromoterCbhAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by