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pAC-85b

Plasmid

#53282

Purpose

Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, crtY, crtI

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTol2-elavl3-Voltron-ST

Plasmid

#119038

Purpose

Pan neuronal expression of soma-localized Voltron in zebrafish

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Use

Zebrafish expression

Promoter

elavl3

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Publication

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRN3P_EGFP_m6A_Tracer

Plasmid

#139403

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

m6A-Tracer

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pETM33_Nsp15

Plasmid

#156481

Purpose

Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT/FLAG/HA-DEST LIN28B

Plasmid

#43798

Depositor

Thomas Tuschl

Publication

Hafner et al RNA. 2013 May;19(5):613-26. doi: 10.1261

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LIN28B (LIN28B Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DN9

Plasmid

#21209

Depositor

Paul Khavari

Publication

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Mouse, Human)

Use

Subclone vector

Mutation

Mek1 K97M fused to ER*, catalytically inactive

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag-ECFP-GIT1

Plasmid

#15223

Depositor

Rick Horwitz

Publication

Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

ECFP and flag

Expression

Mammalian

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pZmUBQ:LUC

Plasmid

#131860

Purpose

Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Publication

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

luciferase

Expression

Plant

Promoter

ZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Publication

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

John Doench, David Root

Publication

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYUB1062-GFP

Plasmid

#117695

Purpose

Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

Depositor

Elizabeth Fullam

Publication

Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

Type

Empty backbone

Tags

GFP and His

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Beclin1

Plasmid

#99574

Purpose

Expressed Cas9 with sgRNA targeting Beclin1

Depositor

Edward Campbell

Publication

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting Beclin1 (BECN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQTEV-STX6

Plasmid

#31581

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

syntaxin 6 (STX6 Human)

Tags

His and TEV

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Publication

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-WHaloCaMP1a-EGFP

Plasmid

#205313

Purpose

Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WHaloCaMP1a-EGFP

Expression

Mammalian

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Publication

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Publication

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Tags

GST and PSP

Expression

Bacterial

Promoter

tac

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Publication

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Expression

Bacterial

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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Bsrs078-LasR

Plasmid

#85147

Purpose

Constitutively expresses LasR

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Insert

LasR

Available since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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