4,177 results

1. pIS0

   Plasmid

   #12178

   Purpose

   3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

   Depositor

   David Bartel

   Article

   Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV/TO Puro DEST (670-1)

   Plasmid

   #17293

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-rab9 WT

   Plasmid

   #12663

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB9 (Homo sapiens) (RAB9A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. HDAC5 Flag

   Plasmid

   #13822

   Purpose

   Mammalian expression of human histone deacetylase 5 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC5 (HDAC5 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR4-24XMS2SL-stable

   Plasmid

   #31865

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   24xMS2 Stem Loops - Stable
   

   Available Since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-Puro-BRAF-V600E

   Plasmid

   #15269

   Purpose

   Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Valine 600 to Glutamate (mutation has been found …

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-cdk5GFP

   Plasmid

   #1346

   Depositor

   Li-Huei Tsai

   Insert

   CDK5 (CDK5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZL Neo Myr Flag TBK1

   Plasmid

   #20648

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSCMV:tdTomato

    Plasmid

    #30530

    Purpose

    mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

    Has Service

    Cloning Grade DNA

    Insert

    tdTomato
    

    Expression

    Mammalian

    Mutation

    R415H

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV/D374Y-hPCSK9

    Plasmid

    #58379

    Purpose

    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Insert

    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    D374Y

    Promoter

    HCRApoE/hAAT

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. IRE1 alpha-pcDNA3.EGFP

    Plasmid

    #13009

    Depositor

    Fumihiko Urano

    Article

    Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

    Insert

    Inositol Requiring Enzyme-1 (ERN1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-AMOT p130

    Plasmid

    #32821

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    E5D

    Promoter

    CMV

    Available Since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Kir2.1-T2A-tdTomato

    Plasmid

    #60598

    Purpose

    Expresses Kir2.1-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Klotho (membrane)

    Plasmid

    #17712

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    P101S

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-ASC-HA

    Plasmid

    #41553

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    ASC (PYCARD Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV immediate-early enhancer/promoter

    Available Since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNANOG-Luc

    Plasmid

    #25900

    Depositor

    Ren-he Xu

    Article

    Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

    Insert

    NANOG promoter (NANOG Human)
    

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-BAP1

    Plasmid

    #22539

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX_306

    Plasmid

    #41391

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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