Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
100+ requests Clear filter

Applied Filters

Clear All Filters
100+ requests Clear filter

4,508 results

Showing: 2701 - 2720 of 4508 results

  3. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Publication
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104992-plasmid-map-sequence-id-213507

    Enlarge

  4. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112203-plasmid-map-sequence-id-473446

    Enlarge

  13. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72266-plasmid-map-sequence-id-447561

    Enlarge

  14. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Publication
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63891-plasmid-map-sequence-id-450336

    Enlarge

  15. pCMV CEPIA4mt

    Plasmid
    #58220
    Purpose
    Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)
    Depositor
    Masamitsu Iino
    Publication
    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CEPIA4mt
    Tags
    myc
    Expression
    Mammalian
    Mutation
    GCaMP2 N263Y E282V E334D M339L F395W D436E
    Promoter
    CMV
    Available since
    July 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58220-plasmid-map-sequence-id-274391

    Enlarge

Showing: 2701 - 2720 of 4508 results