We narrowed to 9,268 results for: SUB
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Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMito-LSSmOrange
Plasmid#37135DepositorInsertLSSmOrange
UseTagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorInsertcsgA (csgA )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgB
Plasmid#89582PurposeExpress csgB curli protein with GS linker and HistaqDepositorInsertcsgB (csgB )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsFKBP-GFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1alpha
Plasmid#44224DepositorInsertPP1A (PPP1CA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
UseTags3xFLAG-tag and His14-tagExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EGFP
Plasmid#19010DepositorInsertp40phox (NCF4 Human)
UseTagsEGFPExpressionMammalianMutationaa1-148 (PX domains)PromoterCMVAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG
Plasmid#211549PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1gamma
Plasmid#44225DepositorInsertPP-1G (PPP1CC Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEXSY-hyg2.1_LtIFT43_Flag
Plasmid#194433PurposeExpression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolaeDepositorInsertIntraflagellar transport complex subunit 43
UseLeishmania tarentolae expressionTags3X Flag and LinkerExpressionMutationPromoterL.donovani promoter elementsAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-4mtD3cpv
Plasmid#36324DepositorInsert4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
UseTagsFirst 29 aa of human COX subunit 8aExpressionMutationPromoterCMVAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer P-null replicon
Plasmid#65462Purposecontaining 1.3 units of the P-null HBV genome (subtype ayw)DepositorInsert1.3 units of the P-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cherry-ICP0-C3
Plasmid#134560PurposeExpresses Cherry-ICP0 fusion proteinDepositorInsertICP0
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/Rabaptin-5:R5BD
Plasmid#168808PurposeProduction of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily membersDepositorInsertRab5-binding domain (aa739-862) of Rabaptin-5
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61β
Plasmid#172445PurposeExpression of ER protein Sec61beta with an N-terminal mCherry tagDepositorInsertProtein transport protein Sec61 subunit beta
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS405-FlagPEN2/PS1wt
Plasmid#207803PurposeYeast integrative vector for expression of FlagPEN2 and Presenilin 1DepositorInsertGamma-secretase subunits PEN-2 and presenilin1 wt
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-rtTR-KRAB-2SM2
Plasmid#11779PurposeTet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET16_Caspase-4_p10
Plasmid#214308PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_p20_C258A
Plasmid#214307PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationC258APromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-FnCpf1
Plasmid#129554PurposeContains codon-optimized FnCpf1 and gRNA for streptomycesDepositorInsertFnCpf1
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB-2SM2
Plasmid#11652PurposeTet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
CFP-tTA-ER-C3
Plasmid#134569PurposeExpresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion proteinDepositorInserttTA-ER
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG015 (RADAR)
Plasmid#157893PurposeExpresses the RADAR defense systemDepositorInsertRADAR subtype containing auxillary gene rdrD
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-APC11
Plasmid#19900DepositorInsertAPC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EYFP
Plasmid#19011DepositorInsertp40phox (NCF4 Human)
UseTagsEYFPExpressionMammalianMutationaa1-148 (PX domains)PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40PX(aa1-148)
Plasmid#19008DepositorInsertp40phox (NCF4 Human)
UseTagsGSTExpressionBacterialMutationaa1-148 (PX domains)PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pECFP(C1)-PP1gamma
Plasmid#44232DepositorInsertPP-1G (PPP1CC Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pQTEV-PPP1R1A
Plasmid#31331DepositorInsertprotein phosphatase 1 (PPP1R1AP1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-2mCh-PS
Plasmid#112268PurposeIDOCKS Acceptor ConstructDepositorInserttwo copies of mCherry followed by a pseudosubstrate peptide for PKC
UseTagsmChExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEYFP(C1)-PP1alpha
Plasmid#44231DepositorInsertPP1A (PPP1CA Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD11
Plasmid#31333DepositorInsertproteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-SNAP-CLIP-GB1a
Plasmid#125709PurposeEukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptorDepositorInsertSNAP-CLIP-GB1a
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMito-iRFP720
Plasmid#45464DepositorInsertiRFP720
UseTagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationT2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Synthetic, Nematode)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-Rabaptin-5
Plasmid#199173PurposeExpresses GFP-Rabaptin-5 fusion in mammalian cellsDepositorInsertRabaptin-5 (RABEP1 Human)
UseTagsEGFPExpressionMammalianMutationContains Tyr 149 Ser substitution; insert starts …PromoterCMVAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac2(V12)
Plasmid#11397DepositorInsertras-related C3 botulinum toxin substrate 2 (RAC2 Human)
UseTagsYFPExpressionMammalianMutationObtained with Glycine 12 changed to Valine.PromoterAvailable sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGGF012
Plasmid#48849PurposeProvides a sulfadiazine resistance cassette as GreenGate module.DepositorInsertpMAS::SulfadiazineR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition sites in SulfR removed …PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40R57Q PX
Plasmid#19009DepositorInsertp40phox (NCF4 Human)
UseTagsGSTExpressionBacterialMutationaa1-148 (PX domains) site directed mutagenesis: …PromoterAvailable sinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only