We narrowed to 2,785 results for: ALC

1. pGP-CMV-GCaMP6-210

   Plasmid

   #91778

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-210
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSYC-437

   Plasmid

   #163829

   Purpose

   Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

   Depositor

   Seok-Yong Choi

   Article

   Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

   Inserts

   Foxj1a Promoter
   GCaMP6s
   

   Use

   Multisite gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-CMV 3xFLAG-LC3A-GFP

   Plasmid

   #123106

   Purpose

   Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

   Plasmid

   #119723

   Purpose

   AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Thomas Oertner

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Inserts

   CaMPARI2
   CaMPARI2
   

   Use

   AAV

   Tags

   NES-his

   Expression

   Mammalian

   Mutation

   F391W, L398V

   Promoter

   hSyn1

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GFAP-TurboID

   Plasmid

   #227179

   Purpose

   Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP_short

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CaMKII-TurboID

   Plasmid

   #227178

   Purpose

   Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa_short

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaMKIV-DCTK75E

   Plasmid

   #126423

   Purpose

   A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed lysine 75 to glutamic acid

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

   Plasmid

   #101062

   Purpose

   AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2-H396K
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. actc1b-mKate2-SERCA1

   Plasmid

   #109498

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   SERCA1 (atp2a1 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Expression

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX-IY LC3A

    Plasmid

    #89290

    Purpose

    For expression of the mammalian Atg8 protein LC3A without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3A (MAP1LC3A Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-GcAMP5

    Plasmid

    #126569

    Purpose

    Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    GcAMP5
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.1 R57A, R59A pMT2

    Plasmid

    #206112

    Purpose

    expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. cacna2d1 HA D491A pcDNA3

    Plasmid

    #206070

    Purpose

    expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D491A

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92229

    Purpose

    Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    p70b (p70a promoter with one mutation)

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Use

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-S1-GCaMP6f

    Plasmid

    #160722

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ultra-Exon1Q145-Myc-A

    Plasmid

    #110487

    Purpose

    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

    Depositor

    Baoji Xu

    Article

    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

    Insert

    Homo sapiens huntingtin (HTT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Partial long 3'UTR wild type huntingtin exon…

    Promoter

    UbC

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBoBi-hTRPV4-C-GC6s

    Plasmid

    #154150

    Purpose

    To detect the calcium ion releases from TRPV4 channel

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    TRPV4 and GCamp6s (TRPV4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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