We narrowed to 3,423 results for: mpo

1. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

   Plasmid

   #169163

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

   Plasmid

   #96888

   Purpose

   expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

   Depositor

   Hening Lin

   Article

   Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

   Insert

   Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)
   

   Use

   Tags

   FLAG-tagged

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-36, mutated lysine 351 to ar…

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-AAV1.X1

   Plasmid

   #209780

   Purpose

   non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV1-X1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. rAAV9-CAP-in-cis-Lox-AAV

   Plasmid

   #205990

   Purpose

   rAAV9 cap acceptor vector for CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   rAAV9 cap acceptor vector for CREATE libraries
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Deletion between XbaI and AgeI sites

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-spikeRBD::∆ACE2 SURF

   Plasmid

   #206957

   Purpose

   Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

   Depositor

   Xiaokun Shu

   Article

   Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

   Inserts

   sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
   ACE2 (ACE2 Human)
   

   Use

   Tags

   cSURF, mCherry, and nSURF

   Expression

   Mammalian

   Mutation

   17-615 and RBD (319-541)

   Promoter

   CMV

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCmini-iCAP-AAV.CAP-Mac

   Plasmid

   #200658

   Purpose

   Synthetic construct isolate AAV.CAP-Mac VP1 gene

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   Synthetic construct isolate AAV.CAP-Mac VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   7 amino acid insertion after Q588 of the AAV9 CAP…

   Promoter

   p41

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Use

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EmGFP-LATS2/1 KD

    Plasmid

    #52085

    Purpose

    Lentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporter

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    LATS2 and LATS1 miRNAs (LATS1, LATS2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX303 TagRFP-T LGALS3 R186S

    Plasmid

    #202428

    Purpose

    Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    R186S

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169194

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28 MBP-TEV-Rat BCKDK-His

    Plasmid

    #232119

    Purpose

    For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (Bckdk Rat)
    

    Use

    Tags

    6 x His, Maltose-Binding Protein (MBP), and Tev S…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

    Plasmid

    #209199

    Purpose

    Mutagenesis of Kcnma1

    Depositor

    Larry Zweifel

    Article

    Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

    Insert

    Kcnma1 (Kcnma1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-BRAF-REG-T241P-Halo

    Plasmid

    #202549

    Purpose

    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.

    Depositor

    Deborah Morrison

    Article

    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

    Insert

    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Threonine 241 mutated to proline

    Promoter

    CMV

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_ZKSCAN1_Tau10-12K317M

    Plasmid

    #194164

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 WT (MAPT Human)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Lysine 317 to Methionine

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGG_Sr1644-1Sh

    Plasmid

    #164087

    Purpose

    Expresses pGGG_Sr1644-1Sh in plants

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

    Insert

    pGGG_Sr-1644-1Sh
    

    Use

    Tags

    none

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Native promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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