We narrowed to 11,926 results for: pcDNA

1. pcDNA3.1(+)-Golgi-RasAR

   Plasmid

   #205570

   Purpose

   Golgi membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   Golgi-RasAR (RAF1, GOLGB1 Synthetic, Human)
   

   Use

   Tags

   Golgin B1 (Giantin) residues 3131-3259

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 hSCN5A-L325R

   Plasmid

   #145373

   Purpose

   expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 325 to Arginine

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY127 (pcDNA3.1-U6-huTsCpf1)

   Plasmid

   #92303

   Purpose

   Expression of humanized TsCpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/TO-Ataxin3-Strep-HA

   Plasmid

   #113496

   Purpose

   Expression of human Ataxin-3 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT-ChemoG1

   Plasmid

   #193806

   Purpose

   CMV overexpression of ChemoG1 in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG1
   

   Use

   Tags

   10xHis

   Expression

   Mammalian

   Mutation

   EGFP: A206K

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-FLAG-GPD1L

   Plasmid

   #32486

   Depositor

   Pere Puigserver

   Article

   Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

   Insert

   GPD1L (Gpd1l Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1/V5-His-TN-DROSHA

   Plasmid

   #106252

   Purpose

   Express TN-DROSHA in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

   Insert

   Drosha Ribonuclease III (DROSHA Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   E1045Q and E1222Q mutations

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-RsSHC014-RBD-8h

   Plasmid

   #161826

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag Jnk1b1

   Plasmid

   #13753

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b1 (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+)-RABIF-FLAG

    Plasmid

    #135558

    Purpose

    Express human RABIF in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Q769gp120-8his

    Plasmid

    #100929

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6.MCV.LT206.V5(CM2B4)

    Plasmid

    #28190

    Depositor

    Patrick Moore

    Insert

    MCPyV genomic T antigen locus (206 wild-type strain)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-neo-Strep_Flag_Cterm

    Plasmid

    #102645

    Purpose

    Epitope tag c-terminus with SBP-Flag moiety

    Depositor

    Michael E. Wright

    Article

    Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 EYFP H148Q

    Plasmid

    #25871

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with Histidine 148 to …

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-HisFlag-CAD

    Plasmid

    #31615

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

    Insert

    CAD
    

    Use

    Tags

    3xFlag, His, and T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-MS2-HA-HsMex3B_AD

    Plasmid

    #148478

    Purpose

    Mammalian Expression of HsMex3B

    Depositor

    Elisa Izaurralde

    Insert

    HsMex3B (MEX3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MS2-HA-HsUNR_AA

    Plasmid

    #148239

    Purpose

    Mammalian Expression of HsUNR

    Depositor

    Elisa Izaurralde

    Insert

    HsUNR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Lunatic Fringe

    Plasmid

    #24962

    Depositor

    Edward De Robertis

    Article

    Pera et al Int J Dev Biol. 2005 . 49(7):781-96.

    Insert

    Lunatic Fringe (lfng Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only