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LLP769_L2pV1_NOR_B3RT_FRT_Rluc_1IF_lacZ

Plasmid

#192429

Purpose

To act as a 1-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP771_L2pV1_NOR_B3RT_FRT_Rluc_2IFB_lacZ

Plasmid

#192431

Purpose

To act as a 2-input state of the NOR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-Rluc-B3RT-FRT

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP759_L2pV1_OR-OCS_0I

Plasmid

#192418

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP760_L2pV1_OR-OCS_TCTP-Flp

Plasmid

#192419

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP766_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IBF_lacZ

Plasmid

#192410

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP767_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_2IFB_lacZ

Plasmid

#192411

Purpose

To act as a 2-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP763_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_0I_lacZ

Plasmid

#192407

Purpose

To act as the 0-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ

Plasmid

#192383

Purpose

To act as the off state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

Plasmid

#192384

Purpose

To act as the on state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ

Plasmid

#192416

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ

Plasmid

#192417

Purpose

To act as the 2-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ

Plasmid

#192415

Purpose

To act as a 1-input state of the split-NAND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

FRT-Act2-FRT::RlucRluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1

Plasmid

#192414

Purpose

To act as the 2-input state of the split-AND gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP764_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_1IB_lacZ

Plasmid

#192408

Purpose

To act as a 1-input state of the A NIMPLY B gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

B3RT-Act2-B3RT::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9341 (pgRNA_IX-1_NatMX)

Plasmid

#161593

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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MTK4a_021

Plasmid

#123834

Purpose

Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

T2A::NLS::mRuby2::PEST4d

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_d2eGFP

Plasmid

#26821

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-d2eGFP-3'UTR

Expression

Mammalian

Mutation

destabilized (PEST sequence) GFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Expression

Mammalian

Promoter

Ef1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.AX

Plasmid

#195218

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

Depositor

Viviana Gradinaru

Article

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

Insert

Synthetic construct isolate AAV-PHP.AX VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgSTAT3-1

Plasmid

#121425

Purpose

sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgSTAT3-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) 12xHRE-NanoLuc

Plasmid

#208575

Purpose

hypoxic response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
12xHRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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HIV-dual-GT

Plasmid

#122696

Purpose

Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

Depositor

Klaus Überla

Article

Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

Inserts

mTagBFP2
DsRed-max

Use

Lentiviral

Tags

PEST domain

Promoter

HIV-1 LTR

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2_Firefly_degron-60A

Plasmid

#200396

Purpose

A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase - degron

Expression

Mammalian

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag(22aa)

Plasmid

#215745

Purpose

Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

dCas9-10xSunTag

Expression

Mammalian

Promoter

chicken beta-actin promoter

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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OA984-HA

Plasmid

#120362

Purpose

Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter

Depositor

Omar Akbari

Article

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 his-tagged single chain antibody

Use

Unspecified

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTK47_mCherry-LGN-hardened

Plasmid

#46347

Purpose

rescues the phenotypes caused by LGN RNAi

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

LGN (GPSM2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTK331

Plasmid

#59572

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgGAL4

Plasmid

#121514

Purpose

sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgGAL4

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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