We narrowed to 13,948 results for: CRISPR-Cas9

Showing: 5441 - 5480 of 13948 results

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pAC-U63-tgRNA-nlsBFP

Plasmid

#169029

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmEGFP-C

Plasmid

#113751

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pOC4 Actb-mRuby3 KI

Plasmid

#183437

Purpose

FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and mRuby3 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 Tubb3-GFP KI

Plasmid

#183440

Purpose

FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ138A

Plasmid

#69280

Purpose

Golden Gate entry vector; 8th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ137A

Plasmid

#69279

Purpose

Golden Gate entry vector;7th gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-3xmRuby-N

Plasmid

#113754

Purpose

Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK198

Plasmid

#65629

Purpose

"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 4xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-mEGFP-C

Plasmid

#113749

Purpose

Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-mRuby-C

Plasmid

#113755

Purpose

Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-3xmRuby-C

Plasmid

#113757

Purpose

Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGMF2-D

Plasmid

#242206

Purpose

AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in dicots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 AtU6-26p::sgRNA2 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU6-26p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT58

Plasmid

#204304

Purpose

Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-DsRed

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-tdTomato166/892

Plasmid

#188488

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-tdTomato90/816

Plasmid

#188487

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rtTA-sg-mGreenLantern/EGFP

Plasmid

#188482

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern/EGFP sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-2xGFP KI

Plasmid

#183443

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 2xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 GFP-Mpp2 KI

Plasmid

#183434

Purpose

CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC5 smFP-HA-Cacna1e KI

Plasmid

#183441

Purpose

FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Gria1-Halo KI

Plasmid

#183433

Purpose

CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and Halo donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Tubb3-3xGFP KI

Plasmid

#183444

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 3xGFP donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Tubb3 no donor

Plasmid

#183431

Purpose

CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 GFP-Actb KI

Plasmid

#183429

Purpose

CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-U63-tgRNA-Gal80

Plasmid

#169030

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
sgiRFP1

Plasmid

#113972

Purpose

Single short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
sgiRFP2

Plasmid

#113973

Purpose

Single short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
sgiRFP3

Plasmid

#113974

Purpose

Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-mRuby-N

Plasmid

#113752

Purpose

Gateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-2xmEGFP-N

Plasmid

#113747

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-3xmEGFP-N

Plasmid

#113748

Purpose

Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

3xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-2xmEGFP-C

Plasmid

#113750

Purpose

Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP-mEGFP

Use

CRISPR; Gateway entry vector

Tags

2xmEGFP

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-R4R3-2xmRuby-N

Plasmid

#113753

Purpose

Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

2xmRuby2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5441 - 5480 of 13948 results

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We narrowed to 13,948 results for: CRISPR-Cas9