We narrowed to 22,374 results for: ESC;

1. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBR322-bom

   Plasmid

   #155178

   Purpose

   pBICI_2_strong without bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046925

   Plasmid

   #145694

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. dCas12j2 VPR

   Plasmid

   #188518

   Purpose

   Expresses FLAG tagged dCas12j2 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D394A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol1-UAS:CheRiff-tdTomato

   Plasmid

   #124234

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   CheRiff-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Promoter

   14xUAS-E1b

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. D3 clone (p21/WAF1 promoter)

   Plasmid

   #16452

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   WAF1 promoter (CDKN1A Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pGST2-CeEP55

   Plasmid

   #21501

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   CEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Q230 (CAG changed to TAA), Q218 (CAA changed to T…

   Available Since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLAMP1-miRFP718nano

   Plasmid

   #195749

   Purpose

   LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   LAMP1-miRFP718nano
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pActin-miRFP718nano

   Plasmid

   #195745

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano-human beta-actin
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBICI_2_strong

    Plasmid

    #155168

    Purpose

    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRE157

    Plasmid

    #118507

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nano-lantern-H2B/pcDNA3

    Plasmid

    #51971

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Tags

    human H2B

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE156

    Plasmid

    #118506

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-syn-fRGECO1

    Plasmid

    #125243

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO1
    

    Expression

    Mammalian

    Mutation

    D323A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p.UTA.2.0-GL-2

    Plasmid

    #82440

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Mutation

    The complementary region for GL2 siRNA control wa…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBW-m4046952

    Plasmid

    #145718

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ERroGFP_C147S_pCDNA3

    Plasmid

    #48630

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C147S

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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