We narrowed to 76,952 results for: rest

1. psiCHECK2-mMOV10-3'UTR-MRE16-mut1

   Plasmid

   #178906

   Purpose

   dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   3'UTR mMov10 (Mov10 Mouse)
   

   Use

   Luciferase

   Mutation

   CTG>AGT miR16-5p MRE1 mutated

   Promoter

   SV40 promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK2-mMOV10-3'UTR-MRE153-mut4

   Plasmid

   #178909

   Purpose

   dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   3'UTR mMov10 (Mov10 Mouse)
   

   Use

   Luciferase

   Mutation

   TGC>CAT miR153-3p MRE mutated

   Promoter

   SV40 promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus

   Plasmid

   #186409

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA

   Plasmid

   #186410

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ST*-GFP

   Plasmid

   #163667

   Purpose

   Expresses partial length ST in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the first 113 amino acids o…

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLyGo-Bs-4

   Plasmid

   #163142

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLyGo-Ec-4

   Plasmid

   #163130

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLyGo-Bs-3

   Plasmid

   #163141

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrichoGate-3

    Plasmid

    #160703

    Purpose

    tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    tef1-alpha
    

    Use

    Synthetic Biology

    Mutation

    Incorporation of BsaI restriction sites

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CTMP

    Plasmid

    #129560

    Purpose

    cytoplasmic marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SMT201

    Plasmid

    #134816

    Purpose

    SMT101 with TET promoter for TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ290-D570A-SRDX

    Plasmid

    #129682

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOVC1_X_AsPT1-LOV2-EcSsra

    Plasmid

    #131217

    Purpose

    Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AsPT1-LOV2-EcSsra
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOVC1_X_AsPT1-LOV2-pep

    Plasmid

    #131215

    Purpose

    Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AsPT1-LOV2-pep
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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