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pJW1673

Plasmid

#112047

Purpose

CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SCT1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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circRAJ31

Plasmid

#69926

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31

Use

Synthetic Biology

Expression

Bacterial

Promoter

PltetO1-2

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-NatMx

Plasmid

#122918

Purpose

ADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1670

Plasmid

#112044

Purpose

CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

OLE1

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

P319T

Promoter

GAL1

Available Since

May 28, 2022

Availability

Academic Institutions and Nonprofits only

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pICE MET17

Plasmid

#122880

Purpose

MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-MtrR

Plasmid

#51188

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

MtrR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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huβB1cry:mAppleCAAX

Plasmid

#122451

Purpose

Human BB1 crystallin promoter driving mApple-CAAX in Tol2 destination vector

Depositor

Thomas Schilling

Article

Vorontsova et al J Vis Exp. 2019 May 6;(147). doi: 10.379

Insert

Human BB1 crystallin:mApple_CAAX

Expression

Bacterial

Promoter

300bp Human Beta B1 crystallin promoter

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-JadR2

Plasmid

#51178

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

JadR2

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-URA3

Plasmid

#122915

Purpose

TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-KanMx

Plasmid

#122917

Purpose

ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-CalR1

Plasmid

#51161

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

CalR1

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_H1237

Plasmid

#64290

Purpose

OmpA redesign hybrid with wild-type strands 1237

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1237

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pET21a-CasR

Plasmid

#51163

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

CasR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HphMx

Plasmid

#122919

Purpose

LYS2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-NatMx

Plasmid

#122921

Purpose

LYS2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_H-3

Plasmid

#64310

Purpose

OmpA redesign hybrid with wild-type strands 1245678

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-3

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LuxT

Plasmid

#51183

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LuxT

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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