We narrowed to 11,615 results for: SHA

1. pDest27-mBIN1+17

   Plasmid

   #61096

   Purpose

   Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. mOrange2-Clathrin-15

   Plasmid

   #57947

   Purpose

   Localization: Clathrin Vesicles, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CaMKII-nirButterfly

   Plasmid

   #136591

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

   Depositor

   Vladislav Verkhusha

   Article

   Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

   Insert

   nirButterfly
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR/U6 HDAC4 shRNA

   Plasmid

   #32220

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   shRNA against murine HDAC4 (Hdac4 Mouse)
   

   Use

   RNAi; Gateway u6 entry vector

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAd FLAG HDAC5 S259A/S498A

   Plasmid

   #32208

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S259A/S498A

   Promoter

   Available since

   Nov. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pENTR/U6 HDAC5 shRNA

   Plasmid

   #32222

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   shRNA against mouse HDAC5 (Hdac5 Mouse)
   

   Use

   RNAi; Gateway u6 entry vector

   Tags

   Expression

   Mutation

   Promoter

   human U6

   Available since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

7. mOrange2-LaminB1-10

   Plasmid

   #57966

   Purpose

   Localization: Nuclear Envelope, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. mOrange2-Keratin-17

   Plasmid

   #57965

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagRFP-T-Fibrillarin-7

   Plasmid

   #58016

   Purpose

   Localization: Nucleoli, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pLL7.0-iRFP670-micro

    Plasmid

    #135954

    Purpose

    Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    iLID micro (SspB R73Q)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKeratin-miRFP703

    Plasmid

    #79990

    Purpose

    Keratin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Hygro-sgTom

    Plasmid

    #138698

    Purpose

    Expresses a tomato-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mOrange2-EB3-7

    Plasmid

    #57953

    Purpose

    Localization: MT End Binding Protein, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pHJ425

    Plasmid

    #20683

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn; and change…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

15. mOrange2-Vimentin-7

    Plasmid

    #57977

    Purpose

    Localization: Intermediate Filaments, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCK-D390TN(UniProt)-FLAG

    Plasmid

    #164693

    Purpose

    Express N-terminally truncated DroshaTN-FLAG in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    N-terminally truncated transdominant negative Drosha (DROSHA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Flag-Trib1

    Plasmid

    #131156

    Purpose

    Mammalian expression of flag-tagged mouse TRIB1

    Depositor

    Karen Keeshan, Warren Pear

    Article

    Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

    Insert

    Trib1 (Trib1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid

    #169992

    Purpose

    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-siriusGFP-FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

    Plasmid

    #172317

    Purpose

    CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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