We narrowed to 23,142 results for: CRISPR

1. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPEY-GAL-Nat

   Plasmid

   #232097

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBlu/gRNA

   Plasmid

   #59188

   Purpose

   Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Guide RNA Cassette
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6 (Arabidopis)

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL31

   Plasmid

   #227428

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

   Depositor

   Pierre Santucci

   Article

   Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

   Insert

   mWasabi
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL32

   Plasmid

   #227429

   Purpose

   Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

   Depositor

   Pierre Santucci

   Article

   Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSQT834

   Plasmid

   #53371

   Purpose

   Csy4 and Cas9 nuclease expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Csy4-T2A-Cas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

    Plasmid

    #64113

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGuide

    Plasmid

    #64711

    Purpose

    Cloning and expression of Streptococcus pyogenes guide RNA

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Streptococcus pyogenes guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ167

    Plasmid

    #69309

    Purpose

    Gateway entry vector with Cas9p

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Expression

    Mutation

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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