We narrowed to 30,451 results for: Ide

1. pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

   Plasmid

   #139844

   Purpose

   Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   AMPK-Kinase-dead (Prkaa2 Rat)
   

   Use

   Lentiviral

   Tags

   GST

   Mutation

   K47R

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. bdSUMO-E5TAT-GFP-HiBit

   Plasmid

   #170993

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-E5TAT-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

   Plasmid

   #176587

   Purpose

   Bacterial expression of His14-MBP-TEV-AmaTnpB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   His14 tag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

7. Circular 100,50 (RAB7A)

   Plasmid

   #170117

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 100,50 guide RNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Circular 200,100 with interspersed loops (SMAD4)

   Plasmid

   #180191

   Purpose

   AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IBU-PFAS-FLAG

    Plasmid

    #168226

    Purpose

    Expresses human PFAS-3xFLAG fusion in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

    Insert

    PFAS (PFAS Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

    Plasmid

    #176537

    Purpose

    Non-protein coding locus components from KraIscB-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from KraIscB-1 locus
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPO685

    Plasmid

    #195300

    Purpose

    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and native array with PaqCI entry site
    

    Expression

    Bacterial

    Promoter

    Arabinose inducible arapBAD

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP2372

    Plasmid

    #73196

    Purpose

    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, and Q926A in SpCas9

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet23a_RBPMS_FL

    Plasmid

    #59392

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIneoLuc-PP1A

    Plasmid

    #128348

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP1A (PPP1CA Human)
    

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. DsRed +9e

    Plasmid

    #191766

    Purpose

    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Tags

    N terminal fusion of positively charged polypetod…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23a_mod_RBPMS_FL

    Plasmid

    #59393

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    6xHis, FLAG, and HA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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