We narrowed to 5,885 results for: dna plasmids

1. pB6_R13-ND51_v8_X1

   Plasmid

   #191512

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE R13-ND51 X1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOD_004

   Plasmid

   #155361

   Purpose

   Gentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRAF-MutL(R95F)

   Plasmid

   #86608

   Purpose

   E. coli Mutl(R95F) expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL(R95F)
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   R95F

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP553‐1

   Plasmid

   #87240

   Purpose

   gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

   Insert

   gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
   

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD06 K. lactis telomerase RNA hairpin

   Plasmid

   #61895

   Purpose

   Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin (nt 229-273)
   

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cdk12_intron4_sg1_pX458

   Plasmid

   #127175

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 4 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneo-hCHD7

   Plasmid

   #89458

   Purpose

   constitutive expression of human CHD7 in mammalian cells

   Depositor

   Peter Scacheri

   Insert

   CHD7 (CHD7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLH1

    Plasmid

    #32859

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1 (MLH1 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    contains amino acids 486-751

    Available Since

    Feb. 22, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. TTN/pAS2-1

    Plasmid

    #98127

    Purpose

    titin in DNA-binding domain (BD) vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Insert

    TTN (TTN Human)
    

    Expression

    Yeast

    Available Since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLgw ELK1Y158A-V5-EcoDam

    Plasmid

    #98609

    Purpose

    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Insert

    ELK1Y158A (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLgw EcoDam-V5-ELK1Y158A

    Plasmid

    #98608

    Purpose

    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Insert

    ELK1Y158A (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Expression

    Plant

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLexA::nud-1

    Plasmid

    #11339

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pLexA::lis-1

    Plasmid

    #11338

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-short_BrokenHeart (no BC)

    Plasmid

    #86950

    Purpose

    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    Brokenheart construct
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. BE3-hA3A-R128A

    Plasmid

    #132944

    Purpose

    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(R128A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-hA3A(R128A)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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