162,309 results

1. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

   Plasmid

   #61628

   Purpose

   Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   ntdTomato
   n2xEGFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   2 copies of EGFP with N-terminal SV40 NLS

   Promoter

   Auxin-dependent DR5 promoter containing 9 reverse…

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-TEAD1

   Plasmid

   #229411

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX_305-C-dTAG

   Plasmid

   #91798

   Purpose

   Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

   Plasmid

   #234826

   Purpose

   STITCHR R2Tocc editor

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   nCas9h840a-xten-R2Tocc
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG94-5

   Plasmid

   #228965

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-5
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-LYC

   Plasmid

   #53270

   Purpose

   Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

   Insert

   crtE, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-flag S127A YAP

   Plasmid

   #27370

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   S127A ( major phosphorylation site in YAP2)

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRLsin-SV40 T antigen-IRES-mCherry

   Plasmid

   #58993

   Purpose

   lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer-chicken beta actin promoter
   SV40 large T antigen
   IRES-mCherry red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   EcoRV site in MCS converted to BamHI site

   Promoter

   CMV enhancer-chicken beta actin

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX_DSB_Spectrum-V1

    Plasmid

    #192474

    Purpose

    Lentiviral DSB Spectrum V1 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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