We narrowed to 4,378 results for: crispr c plasmids

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Little Prince-enOsCas12f1

Plasmid

#251232

Purpose

Drug inducible gene editing system based on enOsCas12f1 (Little Prince)

Depositor

Yu Wang

Article

Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx

Insert

H1-TetO-enOsCas12f1 sgRNA scaffold; Core EF1α-opTtetR; enOsCas12f1; NES; StaPLs-TI; 3×BPNLS; GFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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Little Prince (TnpB)

Plasmid

#251233

Purpose

Drug inducible gene editing system based on TnpB (Little Prince)

Depositor

Yu Wang

Article

Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx

Insert

H1-TetO-TnpB ωRNA* scaffold; Core EF1α-opTtetR; TnpB; NES; StaPLs-TI; 3×BPNLS; GFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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Little Prince (enIscB)

Plasmid

#251234

Purpose

Drug inducible gene editing system based on enIscB (Little Prince)

Depositor

Yu Wang

Article

Zhang et al Sci Transl Med. 2026 May 27;18(851):eadx

Insert

H1-TetO-enIscB ωRNA* scaffold; Core EF1α-opTtetR; enIscB; NES; StaPLs-TI; 3×BPNLS; GFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-SgH

Plasmid

#163972

Purpose

Helper plasmid for cloning gRNAs under the control of the SNR52 promoter.

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

Plasmid

#181973

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; LTBR (LTBR Synthetic, Human)

Use

Lentiviral

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTarget-TTC

Plasmid

#246930

Purpose

for E.coli plasmid interference and PAM depletion assays

Depositor

Caixia Gao

Article

Jin et al Cell. 2025 Sep 29:S0092-8674(25)01035-9.

Insert

TTC PAM and GFP-T1 target

Use

Unspecified

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pX601-Nlgn2-gRNA2-GfaABC1D-mCherry

Plasmid

#250933

Purpose

Nlgn2 gRNA #2 targeting Rat Nlgn2. Also expresses SaCas9 T2a mCherry under the astrocyte promoter gfaABC1D

Depositor

Cagla Eroglu

Article

Sakers et al J Cell Biol. 2026 Jul 6;225(7):e20251211

Insert

Nlgn2 (Nlgn2 Rat)

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pX601-Nlgn2-gRNA3-GfaABC1D-mCherry

Plasmid

#250934

Purpose

Nlgn2 gRNA #3 targeting Rat Nlgn2. Also expresses SaCas9 T2a mCherry under the astrocyte promoter gfaABC1D

Depositor

Cagla Eroglu

Article

Sakers et al J Cell Biol. 2026 Jul 6;225(7):e20251211

Insert

Nlgn2 (Nlgn2 Rat)

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

Plasmid

#185492

Purpose

CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

Depositor

Benjamin Kleinstiver, Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-eGFP

Expression

Mammalian

Mutation

D10A

Promoter

CMV and T7

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pKSB-AeU6-3gRNA

Plasmid

#183914

Purpose

gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

gRNA scaffold for protospacer cloning into BbsI sites

Promoter

Aedes aegypti U6 promoter (AAEL017763)

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-NET1

Plasmid

#232893

Purpose

Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

NET1 gRNA (NET1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-KAE1

Plasmid

#232891

Purpose

Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

KAE1 gRNA (KAE1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VRG4

Plasmid

#232899

Purpose

Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VRG4 gRNA (VRG4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-ARB1

Plasmid

#232888

Purpose

Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

ARB1 gRNA (ARB1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-RER2

Plasmid

#232884

Purpose

Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

RER2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-LEU2-3

Plasmid

#232894

Purpose

Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

LEU2 gRNA (RER2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-HygMx4-URA3-3

Plasmid

#232886

Purpose

Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-HygMx4-URA3-2

Plasmid

#232885

Purpose

Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

URA3 gRNA (URA3 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-AVO1

Plasmid

#232889

Purpose

Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

AVO1 gRNA (AVO1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge