We narrowed to 9,393 results for: Coli

1. pET28-a(+)-6xHis-LSSmCherry1(-13)

   Plasmid

   #219415

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   LSSmCherry1(-13)
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   K137E, K199E

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMAL-c5X-GLP-1

   Plasmid

   #66997

   Purpose

   Expresses malE-GLP-1 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-GLP-1
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28-6His-SUMO-Nsp14

   Plasmid

   #154956

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pG1AK

   Plasmid

   #71736

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIB168

   Plasmid

   #90191

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRL488

   Plasmid

   #70263

   Purpose

   designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.

   Depositor

   Peter Wolk

   Article

   Elhai et al EMBO J. 1990 Oct;9(10):3379-88.

   Type

   Empty backbone

   Use

   Luciferase; Cyanobacteria expression

   Tags

   luxAB

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIB170

   Plasmid

   #90193

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIB164

   Plasmid

   #90187

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcrRNA.con

   Plasmid

   #61285

   Purpose

   Constitutive repeat-only E. coli CRISPR array

   Depositor

   Chase Beisel

   Article

   Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIB167

    Plasmid

    #90190

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIB169

    Plasmid

    #90192

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMAL-c5X-SELP0K

    Plasmid

    #67007

    Purpose

    Expresses malE-SELP0K in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-SELP0K
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIB163

    Plasmid

    #90186

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMAL-c5X-Darpin E_01

    Plasmid

    #66996

    Purpose

    Expresses malE-Darpin E_01 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-Darpin E_01
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKG113

    Plasmid

    #63073

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-T (CENPT Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized. G217R mutation compared …

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-c5X-poly-RGD

    Plasmid

    #67002

    Purpose

    Expresses malE-poly-RGD in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-poly-RGD
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P1 pS43 METTL3

    Plasmid

    #200732

    Purpose

    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3

    Depositor

    Claudio Alarcón

    Insert

    pS43 METTL3 (METTL3 Human)
    

    Use

    Tags

    GST from backbone plasmid

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli, UAG co…

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGL4.26-SS-358

    Plasmid

    #68790

    Purpose

    ZF9 Op x6 -- NTR

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9 Op x6
    Nitroreductase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL-c5X-BRT17

    Plasmid

    #67000

    Purpose

    Expresses malE-BRT17 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-BRT17
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUCX

    Plasmid

    #60681

    Purpose

    E. coli expression plasmid, useful in conjunction with pUCXKT for positive selection cloning and expression

    Depositor

    David Ackerley

    Article

    Prosser et al Biotechnol Lett. 2014 Sep 26.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSETA_ionRE

    Plasmid

    #172926

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUCXKT

    Plasmid

    #60682

    Purpose

    positive selection cloning and expression vector

    Depositor

    David Ackerley

    Article

    Prosser et al Biotechnol Lett. 2014 Sep 26.

    Type

    Empty backbone

    Use

    Positive selection e. coli expression vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-Strep-TEV-Nsp14

    Plasmid

    #154955

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. enz.079-GCN5

    Plasmid

    #154046

    Purpose

    Expresses GCN5 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone acetyltransferase GCN5 (GCN5 Budding Yeast)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRD323

    Plasmid

    #198044

    Purpose

    Expression of HMfA K31A E35A in E. coli

    Depositor

    Remus Dame

    Article

    Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

    Insert

    HMfA K31A E35A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-28a(+)-MVK mazei

    Plasmid

    #108947

    Purpose

    heterologous expression of MVK of M. mazei in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCYP119

    Plasmid

    #50771

    Purpose

    expresses CYP 119 in e. coli

    Depositor

    Stephen Sligar

    Article

    McLean et al Biochem Biophys Res Commun. 1998 Nov 9;2

    Insert

    CYP119
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET-28a(+)-HMGS

    Plasmid

    #108945

    Purpose

    heterologous expression of HMGS of E. faecalis in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    HMG-CoA synthase (HMGS)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GGDestX1-Amp

    Plasmid

    #157652

    Purpose

    Golden Gate assembly and cell-free (bacterial) expression of fusion-proteins

    Depositor

    Karmella Haynes

    Article

    Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

    Type

    Empty backbone

    Use

    Cell-free expression (e. coli sigma-70)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSETA_ionKE

    Plasmid

    #172925

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorKE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMAL-c5X-Dros16

    Plasmid

    #67004

    Purpose

    Expresses malE-Dros16 in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-Dros16
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-28a(+)-MVK jannaschii

    Plasmid

    #108948

    Purpose

    heterologous expression of MVK of M. jannaschii in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Mevalonate kinase (MVK)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pET-28a(+)-IDI2

    Plasmid

    #108951

    Purpose

    heterologous expression of IDI2 of T. thermophilus in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Isopentenyldiphosphat isomerase 2 (IDI2)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX-6P-1-GST-ATL(Cbx7)

    Plasmid

    #82526

    Purpose

    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region only, amino acids 66–81.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ATL(Cbx7), amino acids 66–81

    Promoter

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCVD443

    Plasmid

    #66150

    Purpose

    cloning of EPEC E.coli eae gene

    Depositor

    James Kaper

    Article

    McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

    Insert

    eae
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pET-28a(+)-AACT

    Plasmid

    #108944

    Purpose

    heterologous expression of AACT of M. sedula in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Acetoacetyl-CoA synthase (AACT)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMOD4 RT-G

    Plasmid

    #19184

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    rpsL tetA
    

    Use

    Recombineering

    Tags

    50 nucleotides of FLAG-GFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge