We narrowed to 13,694 results for: NTS

1. pcDNA3.1-SARS-CoV-2-M

   Plasmid

   #215813

   Purpose

   Expresses SARS-CoV-2 Membrane protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Membrane (M )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-SARS-CoV-2-S (D614G)

   Plasmid

   #215838

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSara-C

   Plasmid

   #60722

   Purpose

   Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 180-880 of T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Only amino acids 180-880 of T7 RNAP

   Promoter

   PBAD

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTSara-N

   Plasmid

   #60721

   Purpose

   Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 1-179 of T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Only amino acids 1-179 of T7 RNAP

   Promoter

   PBAD

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP Rootletin (Nigg pFL2(CW499))

   Plasmid

   #41166

   Depositor

   Erich Nigg

   Article

   Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

   Insert

   Rootletin (CROCC Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211842

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   Promoter

   6xUAS-35Smini and pUBQ10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTY07

    Plasmid

    #83285

    Purpose

    human CENP-A N-terminally labeled with LSSmOrange

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    centromere protein A (CENPA Human)
    

    Use

    Retroviral

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

    Plasmid

    #63169

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-FLAG"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Use

    Tags

    T120>A

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LYN11-FRB-CFP

    Plasmid

    #38003

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    LYN (LYN Human)
    MTOR (MTOR )
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pKLV-EF1amCherry-W

    Plasmid

    #159295

    Purpose

    Lentiviral vector expressing mCherry driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB[U6-3 wex2 PUb-DsRed]

    Plasmid

    #169010

    Purpose

    For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    white ex2 sgRNA
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster U6:3

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRII-1.1-2.01 beta

    Plasmid

    #69591

    Purpose

    OT-II TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR beta clone 1.1 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB552

    Plasmid

    #74882

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1-alpha-IIb

    Plasmid

    #27288

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha IIb (ITGA2B Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB511

    Plasmid

    #74853

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-hAsCpf1(TATV) (pY220)

    Plasmid

    #89353

    Purpose

    Expresses humanized AsCpf1 variant that recognizes TATV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CHMP6_GFP

    Plasmid

    #31806

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP6 (CHMP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pXEE401B

    Plasmid

    #91716

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MPRA Empty Vector

    Plasmid

    #204865

    Purpose

    Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tetO-NKX2.5

    Plasmid

    #46029

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    NKX2.5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGWB417

    Plasmid

    #74811

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-4T1 SHP2 WT

    Plasmid

    #8322

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myc-E-Syt3

    Plasmid

    #66835

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt3 (ESYT3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBSFI-myr-akt1

    Plasmid

    #49186

    Purpose

    cDNA of Akt1 with myristyolation signal in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CHMP2A_GFP_N_term

    Plasmid

    #31805

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAT9624-BEAR-cloning

    Plasmid

    #162986

    Purpose

    BEAR target cloning plasmid with split EGFP

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SPOP-V5 (WT)

    Plasmid

    #169720

    Purpose

    Mammalian Expression of wild-type mouse SPOP

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAGM65905

    Plasmid

    #153217

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. dTAG NLRP1

    Plasmid

    #166825

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDIRECT_23A

    Plasmid

    #91138

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGWB541

    Plasmid

    #74875

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge