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mCherry KNL1 wt hardened

Plasmid

#45224

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)

Tags

TEV S15

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-YE1-BE3-FNLS-CMV-mCherry

Plasmid

#154005

Purpose

Express YE1-BE3-FNLS

Depositor

Hui Yang

Article

Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

Insert

YE1-BE3-FNLS

Use

CRISPR

Tags

FLAG

Promoter

CMV

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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TMEM16F-M522K-peGFP-N1

Plasmid

#228579

Purpose

Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

M522K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LV-LIN28A

Plasmid

#117057

Purpose

Overexpression of mouse LIN28A

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

LIN28A (Lin28a Mouse)

Use

Lentiviral

Promoter

hPGK promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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J23104-mRFP1-331Bb

Plasmid

#78274

Purpose

mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23104-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSI2-DCD

Plasmid

#166968

Purpose

Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-DCD

Use

Retroviral

Tags

MSI2-DCD

Expression

Mammalian

Mutation

N495S

Promoter

MSCV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX NM_178152.2, Human)

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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CDK4-3XFlag-pBabe

Plasmid

#73558

Purpose

vector encoding a fusion protein between CDK4 and 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GPR78-Tango

Plasmid

#66374

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR78 (GPR78 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
M87

Plasmid

#242633

Purpose

MYC expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

EGFP-MYC (MYC Human)

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened

Plasmid

#45223

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)

Tags

TEV S15

Mutation

E172G

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

Plasmid

#130976

Purpose

Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

Hook3-SNAPf (HOOK3 Human)

Tags

8His-ZZ-LTLT and SNAPf

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-mCherry-CLCN3

Plasmid

#225514

Purpose

Lentiviral mCherry-CLCN3 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

CLCN3 (CLCN3 Human)

Use

Lentiviral

Tags

mCherry

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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