4,177 results

1. hCathepsin B

   Plasmid

   #11249

   Purpose

   Mammalian expression of human cathepsin B

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin B (CTSB Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM461

   Plasmid

   #108320

   Purpose

   For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   Che9C RecT
   Bxb1 Integrase
   Tet Repressor
   SacRB
   

   Expression

   Bacterial

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Available Since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-LucOS

   Plasmid

   #22777

   Depositor

   Tyler Jacks

   Insert

   OVA-SIY
   

   Use

   Cre/Lox and Lentiviral

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. Emerin pEGFP-C1 (637)

   Plasmid

   #61993

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-hMLN

   Plasmid

   #27079

   Purpose

   Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   C-MYC, LIN28, NANOG (MYC Human)
   

   Expression

   Mammalian

   Available Since

   May 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-Sox2

    Plasmid

    #101761

    Purpose

    Sox2 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SRY-box 2 promoter (SOX2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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