Addgene

We narrowed to 5,770 results for: RIK

Showing: 391 - 420 of 5770 results

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pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

Plasmid

#217760

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C1 domain (aa 123-193)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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5'-3xFLAG Zmynd8-exon minimal CMV pCDNA5

Plasmid

#212078

Purpose

LR vector for integration of Zmynd8-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8-exon (Zmynd8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Msl1-exon minimal CMV pCDNA5

Plasmid

#212068

Purpose

LR vector for integration of Msl1-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Msl1-exon (Msl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Msl1+exon minimal CMV pCDNA5

Plasmid

#212069

Purpose

LR vector for integration of Msl1+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Msl1+exon(48nt) (Msl1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.EPC1

Plasmid

#184653

Purpose

Retroviral expression of mouse Epc1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

Plasmid

#105041

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

Plasmid

#105042

Purpose

Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Rictor (Rictor Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Bcl2

Plasmid

#195551

Purpose

Expresses Bcl2 in mammalian cells

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Bcl2 (Bcl2 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV enhancer and promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-Bcl2-IRES-tdtomato(TET005)

Plasmid

#117857

Purpose

This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Bcl2 (Bcl2 Mouse)

Use

Tta/teto

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#2/Cre

Plasmid

#173648

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPtprd#1/Cre

Plasmid

#173647

Purpose

Expresses a Ptprd-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ptprd (Ptprd Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-Q604L

Plasmid

#79595

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q604L

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-T559N

Plasmid

#79594

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

T559N

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pPRKCZ-C20-EGFP (C-PRKCZ-C20)

Plasmid

#217761

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C20 domain (aa 405-592)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

Plasmid

#217763

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

Plasmid

#217762

Purpose

Fluorescent reporter for ceramide (putative, mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Protein kinase C zeta (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Linker GGSSGGGA plus C20 domain (aa 405-592)

Promoter

CMV

Available since

June 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC-FLAG.Mm.EPC1.delta.EPcA

Plasmid

#184655

Purpose

Retroviral expression of mouse EPC1 delta EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

epc1 delta EPcA

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag.Mm.Epc1-EPcA

Plasmid

#184657

Purpose

Retroviral expression of mouse Epc1 EPcA

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1-EPcA

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.EPC1.delta.EPcC

Plasmid

#184656

Purpose

Retroviral expression of mouse EPC1 delta EPcC

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Epc1 delta EPCc.QT

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Article

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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mouse av full length

Plasmid

#217809

Purpose

Full length mouse integrin av expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGAV (Itgav Mouse)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 ElonginB, ElonginC

Plasmid

#230999

Purpose

Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Elongin B (Elob Mouse)
Elongin C (Eloc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 1

Plasmid

#198489

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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