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Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK462 (Hygro-P2A-mAB)
Plasmid#214388PurposemAID-BromoTag for N-terminal taggingDepositorInsertHygro-P2A-mAID-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS114
Plasmid#215677PurposeEmpty repair plasmid for ChrI split hygromycinR landing padDepositorInsert5'HA + MCS + loxN + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-3xT7-nSREBP1a
Plasmid#171030PurposeExpresses 3xT7-tagged nuclear form of SREBP1a in mammalian cellsDepositorInsertnSREBP1a (Srebf1 Mouse)
UseRetroviralTags3xT7ExpressionMammalianMutationamino acids 1- 449Promoter5' LTRAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK445 (BSD-P2A-BromoTag)
Plasmid#214375PurposeBromoTag for N-terminal taggingDepositorInsertBSD-P2A-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitD
Plasmid#122597Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhyg-3xT7-CA-ChREBP
Plasmid#171029PurposeExpresses 3xT7-tagged constitutive active form of ChREBP in mammalian cellsDepositorInsertCA-ChREBP (Mlxipl Mouse)
UseRetroviralTags3xT7ExpressionMammalianMutationamino acids 240 - 864Promoter5' LTRAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP17_up1691, P20_992, and iP16_3622Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK463 (BSD-P2A-mAB)
Plasmid#214389PurposemAID-BromoTag for N-terminal taggingDepositorInsertBSD-P2A-mAID-BromoTag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorInsertgIII, luxAB, guide RNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK486 (Hygro-P2A-2mAID)
Plasmid#214398Purpose2mAID for C-terminal taggingDepositorInsertHygro-P2A-2mAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK382
Plasmid#214373PurposeTet-OsTIR1(F74G) (AAVS1 insertion donor)DepositorInsertTet-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterTet ON 3G promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#122600PurposeBE PACE selection, express C-intein-Cas9-ugi fusionDepositorInsertintein-dCas9-ugi fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK464 (mAB-Neo)
Plasmid#214390PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BromoTag-Neo
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Mycn
Plasmid#60667PurposePiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoterDepositorInsertMycn (Mycn Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitC
Plasmid#122605Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-StagRFP-AktAR
Plasmid#173020PurposeYellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.DepositorInsertVenus-StagRFP-AktAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-FLuc
Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK467 (dTAG-BromoTag-mAID-EGFP)
Plasmid#214393PurposedTAG-BromoTag-mAID-EGFP reporter (TOL2)DepositorInsertdTAG-BromoTag-mAID-EGFP reporter
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitD
Plasmid#122606Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b
Plasmid#122187PurposeBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-EFSep
Plasmid#34627DepositorInsertEF-Sep (tufB E. coli)
UseTags6xHIS and MBPExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNDGG004
Plasmid#231317PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG053
Plasmid#231322PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-RLuc
Plasmid#188312PurposeExpression of Renilla luciferase via a T7 promoter in TXTLDepositorInsertRenilla luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMK465 (mAB-Hygro)
Plasmid#214391PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BrpmpTag-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK449 (BromoTag-mClover-BSD)
Plasmid#214379PurposeBromoTag-mClover for C-terminal taggingDepositorInsertBromoTag-mClover-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitC
Plasmid#122596Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416 25Q GPD
Plasmid#1177DepositorInserthtt 25Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSExpressionMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only