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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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BasiginCD4d3+4-Comp-His-Stop

Plasmid

#36147

Purpose

EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin (BSG Human)

Use

Tags

His tag, pentamerization domain of rat cartilagin…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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pMT2 Alpha2 delta-1 V6

Plasmid

#89894

Purpose

Mammalian expression of Alpha2 delta-1 V6

Depositor

Annette Dolphin

Article

Kadurin et al Elife. 2016 Oct 26;5. pii: e21143. doi:

Insert

Cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Amino acids L943V, E944V, A945V, E947V, M948V are…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-RECQL5

Plasmid

#222620

Purpose

Mammalian expression vector of flag-tagged RECQL5

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

RECQL5 (RECQL5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-Flag-53BP1

Plasmid

#52507

Purpose

mammalian expression vector of 53BP1

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

V128A and siRNA resistant sequence: ACGCGCTGACGAC…

Promoter

CMV/TO

Available since

March 26, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CD44-isoform1-CD4d3+4-bio

Plasmid

#73098

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD44 antigen isoform 1 (CD44 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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CD44-isoform12-CD4d3+4-bio

Plasmid

#73130

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform12 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD44 antigen isoform 12 (CD44 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-msMMS22L

Plasmid

#221015

Purpose

mammalian expression vector of Flag tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-eGFP-msMMS22L

Plasmid

#221014

Purpose

mammalian expression vector of eGFP tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-Flag-BRIP1

Plasmid

#222619

Purpose

Mammalian expression vector of flag-tagged BRIP1

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

BRIP1 (BRIP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LentiGuide-puro-NLS-mCherry

Plasmid

#185474

Purpose

Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag::UbvG08 I44A, deltaGG

Plasmid

#74939

Purpose

Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Tags

Flag

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available since

June 30, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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LentiGuide-puro-NLS-GFP

Plasmid

#185473

Purpose

Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-eGFP-SHLD1

Plasmid

#114116

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD1

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-eGFP-SHLD2

Plasmid

#114119

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLX304-DCK*-IRES-GFP

Plasmid

#176297

Purpose

Expresses DCK* and GFP from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-IRES-Td

Plasmid

#176298

Purpose

Expresses DCK* and TdTomato from the same transcript

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-GW-DCK*-IRES-GFP

Plasmid

#176291

Purpose

Gateway vector for use in generating POI-DCK* fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pFOXA2

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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