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Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-FLAG-mTET2 (N1041)
Plasmid#89737PurposeExpresses the N1041 truncation to mouse TET2 in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationN-term 1041 truncation of mouse TET2 (contains fi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-sp44/p21
Plasmid#120232PurposeCarries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.DepositorInsertbidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsHaloTagExpressionMammalianMutationDeletion of lumenal domainPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 (N500)
Plasmid#89897PurposeExpresses mouse TET2 N500 truncation in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationN-term 500 truncation of mouse TET2 (contains fir…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-WT
Plasmid#182741PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…TagsExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-mKate-NGFR
Plasmid#104831PurposeextGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detectionDepositorInsertsmKate
NGFR
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-tdTomato
Plasmid#62516PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing proceduresDepositorInserttdTomato
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d4
Plasmid#182743PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 4 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-d13
Plasmid#182745PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform delta 13 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-mCherry
Plasmid#104833Purpose3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detectionDepositorInsertseFFly
mCherry
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-eGFP
Plasmid#62518PurposeExpresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.DepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-p12
Plasmid#182744PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase isoform p12 (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon optimized for expression in homo sapiensPromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iBSD-5LO-E376Q
Plasmid#182742PurposeLentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).DepositorInsert5-Lipoxygenase E376Q (ALOX5 Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationenzymatically inactive 5-LO E376Q mutant, codon o…PromoterSFFVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-MCM3/5
Plasmid#116950PurposeExpresses human MCM3 and human MCM5DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorUseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32-MCM2/7
Plasmid#116949PurposeExpresses human MCM2 and human MCM7DepositorUseTagsThioredoxin, His6ExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only