We narrowed to 4,316 results for: Pet2;

1. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a MDHP_YEAST

   Plasmid

   #204233

   Purpose

   Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHP_YEAST (MDH3 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   The last 3 C-terminal residues (SKL) are the pero…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-FIP3

   Plasmid

   #74753

   Purpose

   Expressed sfGFP-tagged FIP3 in E. coli for protein production

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab11 family interacting protein 3 (RAB11FIP3 Human)
   

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SrtAdelta59

   Plasmid

   #51138

   Purpose

   expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.aureus SrtA delta 59
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Tags

   V5-His

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a hMDH2

   Plasmid

   #204230

   Purpose

   Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH2 (MDH2 Human)
   

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-LIC

   Plasmid

   #26094

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. LCC-ICCG_pET21b

    Plasmid

    #215408

    Purpose

    Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    LCC-ICCG
    

    Expression

    Bacterial

    Mutation

    F243I/D238C/S283C/Y127G

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-KIRIN1

    Plasmid

    #107414

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Expression

    Bacterial

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pet22b-mEGFP

    Plasmid

    #166439

    Purpose

    Bacterial expression of 6x His tagged mEGFP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP
    

    Expression

    Bacterial

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-cMSP26

    Plasmid

    #199769

    Purpose

    Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSP26
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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