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We narrowed to 578 results for: SPY

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Showing: 261 - 280 of 578 results

  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  3. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  8. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid
    #216292
    Purpose
    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-GSG-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216292-plasmid-map-sequence-id-433349

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  11. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

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  13. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid
    #210024
    Purpose
    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    Expression
    Mammalian
    Mutation
    Encephalomyocarditis virus internal ribosome entr…
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210024-plasmid-map-sequence-id-417730

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Showing: 261 - 280 of 578 results