We narrowed to 1,922 results for: cas9 expression vector

1. pMRS-LacI-dCas9

   Plasmid

   #220194

   Purpose

   mini-Tn7 dCas9 expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   LacI/pLac

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9

   Plasmid

   #50915

   Purpose

   Tet-regulatable dCas9 lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRS-AraC-dCas9

   Plasmid

   #220195

   Purpose

   mini-Tn7 dCas9 expression vector - AraC/pBAD promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   AraC/pBAD

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1195-pssAAV.U1a.hNme2Cas9

   Plasmid

   #129534

   Purpose

   AAV vector expressing Nme2Cas9

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Insert

   human codon-optimized Nme2Cas9
   

   Use

   AAV

   Tags

   2xNLS and NLS-3xHA-NLS

   Expression

   Mutation

   Promoter

   U1a

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-L3-Cas9-L2

    Plasmid

    #141038

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-L3-Cas9n-L2

    Plasmid

    #141039

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9n
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. modRNAc1-Cas9-2A-p53DD

    Plasmid

    #216389

    Purpose

    this vector can be used to make modRNA expressing Cas9 and p53DD

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    Cas9-2A-p53DD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. modRNAc1-VP64dCas9VP64-2A-MPH

    Plasmid

    #216388

    Purpose

    this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    VP64dCas9VP64-2A-MPH
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. modRNAc1-Cas9-2A-puro

    Plasmid

    #216390

    Purpose

    this vector can be used to make modRNA expressing Cas9 and puro resistance gene

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    Cas9-2A-puro
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. zCREST3:Cas9green; T3_gRNA

    Plasmid

    #199335

    Purpose

    pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 type III sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. zCREST3:Cas9green; T2_gRNA

    Plasmid

    #197647

    Purpose

    pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 type II sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. zCREST3:Cas9green; T1_gRNA

    Plasmid

    #199332

    Purpose

    pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 type I sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbdCas9G(RBSmut)_arr2

    Plasmid

    #149578

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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