Addgene

Showing: 261 - 280 of 65319 results

Results per page:

Sort results by:

pLViN-iRFP670-α-cateninA+ΔβH

Plasmid

#229707

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-beta catenin tag

Plasmid

#186422

Purpose

Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

GST-beta catenin tag

Use

Tags

6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

Plasmid

#225436

Purpose

BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

Depositor

Diego Orzaez

Insert

BeYDV geminino 1.0

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/110R]

Plasmid

#220384

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/110.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/117R]

Plasmid

#220385

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/117.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/132R]

Plasmid

#220386

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/132.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/133R]

Plasmid

#220387

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/133.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/136R]

Plasmid

#220388

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/136.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/20R]

Plasmid

#220389

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/55R]

Plasmid

#220390

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human) IgG2a R-mAb. Derived from hybridoma L122/55.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-CALB2/calretinin [L122/6R-1]

Plasmid

#206691

Purpose

Mammalian Expression Plasmid of anti-CALB2/calretinin (Human). Derived from hybridoma L122/6-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB2/calretinin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-CALB2/calretinin [L122/6R-2b]

Plasmid

#206642

Purpose

Mammalian Expression Plasmid of anti-CALB2/calretinin (Human). Derived from hybridoma L122/6-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CALB2/calretinin (Homo sapiens) recombinant Mouse monoclonal antibody (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Calretinin scFv [L122/144]

Plasmid

#206780

Purpose

Mammalian Expression of Calretinin scFV. Derived from hybridoma L122/144 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Calretinin (Homo sapiens) recombinant scFV (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Calretinin [L122/144R]

Plasmid

#188159

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human). Derived from hybridoma L122/144

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody (CALB2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_canine-Caspase-1/4_enzymatic-domain

Plasmid

#183383

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC5 cloning template vector

Plasmid

#183424

Purpose

FlpOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOC3 cloning template vector

Plasmid

#183422

Purpose

CreON FlpOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge