We narrowed to 15,050 results for: pET

1. pET28a-anticalin-FLN-ELP-His-ybbr

   Plasmid

   #168039

   Purpose

   Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SdrG-FLN-ELP-His-ybbr

   Plasmid

   #168047

   Purpose

   Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, ddFLN4, and ybbr tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-Synapsin-YPET-3XNLS-optoNES-WPRE

   Plasmid

   #159947

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   YPET-3XNLS-optoNES
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Synapsin-NLS-YPET-optoNES-WPRE

   Plasmid

   #159942

   Purpose

   optical regulation of nuclear export

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

   Plasmid

   #159975

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.F434L
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   F434L

   Promoter

   hSyn1

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

   Plasmid

   #159973

   Purpose

   optical regulation of nuclear export with extended relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.V416I
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V416I

   Promoter

   hSyn1

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

   Plasmid

   #159974

   Purpose

   optical regulation of nuclear export with shorter relaxation time

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   NLS-YPET-optoNES.I427V
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   I427V

   Promoter

   hSyn1

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-45b-TBP(38Q)-IDR-mCherry

   Plasmid

   #145283

   Purpose

   Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   TBP(38Q)-IDR (TBP Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid

    #105738

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    Q79N/Q109N

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid

    #89288

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Asp

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid

    #89289

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Asp

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid

    #87748

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Glu-322 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

    Plasmid

    #61940

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156) GSGS (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 97-100 (N…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET-21a-GFP-Patronin CC 534–868

    Plasmid

    #59054

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXD1 (murine, partial) HIS-tag pET

    Plasmid

    #8560

    Depositor

    Corey Largman

    Insert

    HOXD1 (Hoxd1 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Partial protein containing 10 AA N-terminal to HD…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

21. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

    Plasmid

    #111267

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.5 AB (361-545, Δ395-511)

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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22. Pet28a His6-HSPB7 (zebrafish) C49S/C117S

    Plasmid

    #110073

    Purpose

    Express C49S/C117S double mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 49 to Serine, Cysteine 117 to Serine

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid

    #29655

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid

    #29659

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid

    #29656

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid

    #218973

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Expression

    Mammalian

    Mutation

    mutant SRSF1 binding motif (AGTCTCCT)

    Promoter

    RSV LTR

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid

    #114070

    Purpose

    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge