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Showing: 5561 - 5600 of 8357 results

  2. pLKO-Tet-On-ARNTL-shRNA3

    Plasmid
    #166915
    Purpose
    Lentivirus for inducible knockdown of human ARNTL(BMAL1)
    Depositor
    Roland Friedel
    Insert
    ARNTL shRNA (BMAL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166915-plasmid-map-sequence-id-331234

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  3. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51094
    Purpose
    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51094-plasmid-map-sequence-id-79315

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  4. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  7. pLV CMV IGF1-FLAG

    Plasmid
    #175170
    Purpose
    Lentiviral expression of human Igf1-FLAG
    Depositor
    Larry Gerace
    Insert
    Igf1 (IGF1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    175170-plasmid-map-sequence-id-352639

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  8. pCS Omomyc-Mer

    Plasmid
    #113170
    Purpose
    Expression of Omomer (tamoxifen inducible Omomyc) in mammalian cells
    Depositor
    Sergio Nasi
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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  11. GFP-ESR2

    Plasmid
    #65212
    Purpose
    Full length mammalian expression vector for hESR2
    Depositor
    Ratna Vadlamudi
    Insert
    ESR2 (ESR2 Human)
    Use
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. BRDT

    Plasmid
    #38898
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRDT (BRDT Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. Flag-mFoxJ1

    Plasmid
    #122965
    Purpose
    Expresses Flag-tagged mouse FoxJ1 in mammalian cells
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Insert
    mouse FoxJ1 (Foxj1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    122965-plasmid-map-sequence-id-238008

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  14. FLAG-hPAX3 (#395)

    Plasmid
    #78335
    Purpose
    Overexpression of human PAX3 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    PAX3 (PAX3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pLEX307-TMPRSS2-G418

    Plasmid
    #158459
    Purpose
    Constitutive lentiviral expression of human TMPRSS2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS2 (TMPRSS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    158459-plasmid-map-sequence-id-311225

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  16. FH

    Plasmid
    #38978
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FH (FH Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

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  18. pNY-345

    Plasmid
    #231045
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to rabbit Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231045-plasmid-map-sequence-id-457177

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  19. pNY-347

    Plasmid
    #231047
    Purpose
    Used for GFP staining
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to mouse Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231047-plasmid-map-sequence-id-457176

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  21. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  23. CD55-full length

    Plasmid
    #220874
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 (CD55 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMW
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220874-plasmid-map-sequence-id-443039

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  24. pLEX307-ACE2-puro

    Plasmid
    #158448
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158448-plasmid-map-sequence-id-308163

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  25. pLEX307-ACE2-blast

    Plasmid
    #158449
    Purpose
    Constitutive lentiviral expression of human ACE2.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158449-plasmid-map-sequence-id-434046

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  26. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV420H2 (KMT5C Human)
    Use
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. BRD9

    Plasmid
    #39012
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD9 (BRD9 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  29. pFRT-TODestFLAGHA_HuC

    Plasmid
    #65756
    Purpose
    expresses FLAGHA-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65756-plasmid-map-sequence-id-111826

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  30. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Promoter
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  31. pDONR221-ACE2

    Plasmid
    #158438
    Purpose
    Gateway Cloning compatible entry vector for the human ACE2 gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    ACE2 (ACE2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158438-plasmid-map-sequence-id-308248

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  32. RIX-F2Oct4F3-GFP

    Plasmid
    #216848
    Purpose
    This plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diff
    Depositor
    Patrick Salmon
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Oct4
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216848-plasmid-map-sequence-id-431658

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  33. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid
    #182896
    Purpose
    Mammalian expression of TH mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) heavy chain (TH Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182896-plasmid-map-sequence-id-360557

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  34. pFRT-TODestGFP_HuC

    Plasmid
    #65762
    Purpose
    expresses GFP-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65762-plasmid-map-sequence-id-111834

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  35. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  36. KIF3A406

    Plasmid
    #129768
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    129768-plasmid-map-sequence-id-271499

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  37. pcDNA_DiCre2_60.F2

    Plasmid
    #60210
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 60-343 …
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60210-plasmid-map-sequence-id-96296

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  38. GAK

    Plasmid
    #39042
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAK (GAK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  40. TH mAb_BCN53.1.1D10_HC_Rat IgG2a

    Plasmid
    #182898
    Purpose
    Mammalian expression of TH rat mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) light chain (TH Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182898-plasmid-map-sequence-id-360570

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Showing: 5561 - 5600 of 8357 results