We narrowed to 7,345 results for: Lif

1. ACE2-Fc

   Plasmid

   #164222

   Purpose

   Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 extracellular domain (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

   Plasmid

   #173886

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-Bcl-2-s2193

   Plasmid

   #166750

   Purpose

   Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   Human ferritin

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

   Plasmid

   #188634

   Purpose

   HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Synthetic, Human)
   

   Use

   CRISPR

   Tags

   CD3ε-mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a_DIO_mMeCP2-HaloTag

   Plasmid

   #164062

   Purpose

   Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

   Depositor

   Nathaniel Heintz

   Article

   Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

   Insert

   MeCP2 Isoform 1 (Mecp2 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

   Plasmid

   #173887

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-Gfi1

   Plasmid

   #158531

   Purpose

   Expresses human Gfi1 in mammalian cells

   Depositor

   Neil Segil

   Article

   Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

   Insert

   Growth Factor Independent Protein 1 (GFI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-RNF51

   Plasmid

   #126546

   Purpose

   Expresses GFP-tagged RNF51 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RNF51 (BMI1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-tBid-pEGFP-C1

   Plasmid

   #166736

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   tBid (Bid Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167943

    Purpose

    SFTPC reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SFTPC (SFTPC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    EF1a

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid

    #191093

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B and mScarlet
    

    Use

    AAV

    Tags

    H2B-V5-mScarlet

    Expression

    Mammalian

    Mutation

    The fusion protein was optimized to the Human cod…

    Promoter

    CMV and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPBbsr2-Necl-5-ScNeo

    Plasmid

    #170283

    Purpose

    To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)
    

    Use

    Tags

    mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-VGlut1 [N28/9R]

    Plasmid

    #128638

    Purpose

    Mammalian Expression Plasmid of anti-VGlut1 (Rat). Derived from hybridoma N28/9.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-VGlut1 (Rat) recombinant mouse monoclonal antibody (Slc17a7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-cT3G-cERP2-TSC22D3

    Plasmid

    #192916

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TSC22D3 (TSC22D3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lrrc4c-Fc-His

    Plasmid

    #72085

    Purpose

    Expresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4c (Lrrc4c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. iSH-iRFP670-sspB

    Plasmid

    #176102

    Purpose

    Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    phosphatidylinositol 3-kinase (Pik3r1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Venus-Bad-pEGFP-C1

    Plasmid

    #166733

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bad (BAD Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEB1N-mScarlet-LZ-LOV2

    Plasmid

    #197419

    Purpose

    Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGPN-ACE2

    Plasmid

    #187483

    Purpose

    Expression vector - ACE2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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