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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,181 results for: nes

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Showing: 2781 - 2800 of 16181 results

  3. HUWE1

    Plasmid
    #25211
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    huwe1.4005.4374 (HUWE1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    HECT domain
    Available since
    Feb. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  5. mMaroon-N1

    Plasmid
    #54554
    Purpose
    Localization: N1 Cloning Vector, Excitation: 610, Emission: 652
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mMaroon
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    54554-plasmid-map-sequence-id-475388

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  6. PS-CFP2-Lifeact-7

    Plasmid
    #54695
    Purpose
    Localization: Actin, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-PS-CFP2
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Publication
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  12. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  13. mPA-GFP-Lifeact-7

    Plasmid
    #54671
    Purpose
    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-mPA-GFP
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Publication
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway Cloning
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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  15. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid
    #113862
    Purpose
    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    113862-plasmid-map-sequence-id-328796

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  18. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Publication
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

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Showing: 2781 - 2800 of 16181 results