We narrowed to 23,988 results for: nes

1. pMZ0002

   Plasmid

   #102239

   Purpose

   Expresses Chinese hamster NSF in bacteria

   Depositor

   Axel Brunger

   Article

   Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

   Insert

   N-ethylmaleimide sensitive factor
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

   Plasmid

   #50030

   Purpose

   Expression of cytosolic S. pyogenes SrtA in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. mpCHECK2

   Plasmid

   #167576

   Purpose

   The mpCHECK2 plasmid has the ability to evaluate the effect of polyadenylation signal variants on expression of disease-associated genes.

   Depositor

   Ting Ni

   Article

   Chen et al Genome Res. 2021 Apr 19. pii: gr.270256.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Thy1-Brainbow-2.1 R

   Plasmid

   #18727

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-Brainbow-1.0 H

   Plasmid

   #18720

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   tdimer2 ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-BRIC

   Plasmid

   #172343

   Purpose

   Bioluminescent Red Indicator for Calcium (bacterial expression)

   Depositor

   Huiwang Ai

   Article

   Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

   Insert

   BRIC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Thy1-Brainbow-1.1 M

   Plasmid

   #18726

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV FLIP FF

   Plasmid

   #19744

   Depositor

   Richard Hynes

   Article

   Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   oligo targeting Firefly luciferase
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TEV-VPR

   Plasmid

   #110200

   Purpose

   Encodes for HIV-1 VPR fused with TEV protease (TEVp)

   Depositor

   Sergi Padilla Parra

   Article

   Jones et al Sci Rep. 2015 Aug 24;5:13449. doi: 10.10

   Insert

   VPR, TEVp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TBRE-TK-Luc-WT

    Plasmid

    #36246

    Depositor

    Bruce Blumberg

    Article

    Janesick et al Development. 2012 Mar;139(6):1213-24.

    Insert

    Two copies of the Brachyury T-box site
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    TK minimal promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177433

    Purpose

    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Thy1-Brainbow-1.0 L

    Plasmid

    #18725

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    dTomato ; EYFP ; mCerulean
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pV1081

    Plasmid

    #111425

    Purpose

    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Thy1-Brainbow-1.0 H

    Plasmid

    #18724

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    tdimer2 ; M-mEYFP ; M-mCerulean
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAIO3

    Plasmid

    #137844

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJ163

    Plasmid

    #175056

    Purpose

    rox flanked gentamycin resistance cassette integrates into A2542 integration site

    Depositor

    David Nielsen

    Article

    Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

    Insert

    rox-mR-rox
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS303-pGAL-HA-SrtAstrep (pKS78)

    Plasmid

    #50029

    Purpose

    Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    HA-SrtA(strep)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    pGAL

    Available since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX ALIX-GFP S718/721/E

    Plasmid

    #138584

    Purpose

    Expresses fluorescent ALIX S718/721E in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    changed serines 718 and 721 to glutamic acids

    Promoter

    TREtight

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJ164

    Plasmid

    #175057

    Purpose

    rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL

    Depositor

    David Nielsen

    Article

    Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

    Insert

    DRE
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    rbcLXS

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Venus-79aa-ActA-pEGFP-C1

    Plasmid

    #166763

    Purpose

    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    ActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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