We narrowed to 23,573 results for: CHI

1. pMAL-EFSep

   Plasmid

   #34627

   Depositor

   Dieter Söll, Jesse Rinehart

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   EF-Sep (tufB E. coli)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Available since

   March 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. Luc-SIRT1 3'UTR

   Plasmid

   #20379

   Depositor

   Charles Lowenstein

   Article

   Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   3'UTR of SIRT1

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pTJV1Sc3-rpoB

   Plasmid

   #166983

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   RecT (recT E. coli)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pVvanCC

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPy-CAG-Cre::ERT2-IRES-BSD

   Plasmid

   #48760

   Purpose

   CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   Cre::ERT2
   

   Use

   Ppy

   Tags

   ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nls-Flamindo2

   Plasmid

   #73939

   Purpose

   Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   nls-Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBlueScript-humanAPOBEC1

   Plasmid

   #134606

   Purpose

   Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   humanAPOBEC1 (APOBEC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Beta actin

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046852

   Plasmid

   #145584

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp8

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pKin1B

   Plasmid

   #31604

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   kinesin-1B (Kif5b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a His tag-FRB-Venus C

   Plasmid

   #100980

   Purpose

   Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

   Depositor

   Heinrich Leonhardt

   Article

   Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

   Insert

   His-FRB-Venus C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR1A-3XFlag-WT-MEFV

    Plasmid

    #167018

    Purpose

    Gateway entry plasmid with 3xflag-pyrin (Wild-type)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-DN-14-3-3 zeta (R56A/R60A)

    Plasmid

    #116889

    Purpose

    Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMRX-INU-FLAG-FAM134Bs

    Plasmid

    #128261

    Purpose

    Expresses FAM134Bs in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134Bs (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KA1153_pPBCAG-MerCreMer-IN

    Plasmid

    #124183

    Purpose

    piggyBac-based expression of the tamoxifen-inducible Cre

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    MerCreMer-IRES-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046979

    Plasmid

    #145611

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pRSET FLII12Pglu-700uDelta6

    Plasmid

    #13568

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Feb. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CAG-loxP-3xpA-loxP-H2B-sfGFP

    Plasmid

    #136064

    Purpose

    H2B-sfGFP cre reporter

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    HIST1H2BB (H2BC3 Human)
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046920

    Plasmid

    #145717

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RIB VP64 R1

    Plasmid

    #161493

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGBW-m4046760

    Plasmid

    #145600

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA3-HA-BRAT1

    Plasmid

    #62802

    Purpose

    Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

    Depositor

    Toru Ouchi

    Article

    So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

    Insert

    BRAT1 (BRAT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

23. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKin1A

    Plasmid

    #31607

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGBW-m4046814

    Plasmid

    #145601

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pcDNA4-GFP-Metavinculin

    Plasmid

    #107151

    Purpose

    mammalian expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Proc Natl Acad Sci U S A. 2016 Aug 23;11

    Insert

    metavinculin (VCL Human)
    

    Use

    Tags

    (His)10-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHL 246

    Plasmid

    #53014

    Purpose

    AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

    Depositor

    Han Lim

    Article

    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    fimS (off orientation)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

28. Sender plasmid

    Plasmid

    #127677

    Purpose

    Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-Mycn-IRES-RFP

    Plasmid

    #35393

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

    Inserts

    Mycn (Mycn Mouse)
    RFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2013

    Availability

    Academic Institutions and Nonprofits only