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We narrowed to 4,241 results for: pes;

Showing: 4201 - 4230 of 4241 results
  1. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Promoter
    None
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid
    #123276
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations; …
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available Since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pCMV-αIFNα-ab

    Plasmid
    #110799
    Purpose
    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Insert
    antibody against mouse interferon alpha
    Tags
    ER signal peptide, His6 tag, and myc epitope tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pCMV-αIFNα-ib

    Plasmid
    #110798
    Purpose
    Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Insert
    intrabody against mouse interferon alpha
    Tags
    ER retention signal (SEKDEL), ER signal peptide, …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid
    #156421
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid
    #156418
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. rd-his

    Plasmid
    #112911
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
    Depositor
    Insert
    dematin (DMTN Human)
    Tags
    6-his tag, Glutathione-S-Transferase, and preciss…
    Expression
    Bacterial
    Mutation
    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. NST3_pOpOn2.1

    Plasmid
    #228066
    Purpose
    Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3
    Available Since
    Jan. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Inserts
    PPT1
    fsr1
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. pCEP4-BG505.SOSIP-gp160-QES.i05.c06-754*

    Plasmid
    #123275
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations; …
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pAAVtri-CMV-eGFP

    Plasmid
    #230932
    Purpose
    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.
    Depositor
    Insert
    eGFP
    Use
    AAV
    Promoter
    CMV
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 4201 - 4230 of 4241 results