We narrowed to 8,780 results for: construct
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Plasmid#58476PurposeExpresses a nuclear-targeted mCherry control construct, on a CMV reporterDepositorInsert3X NLS with 3X stop codons
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSynapsin SV-tag
Plasmid#163685Purposeexpress SV-Tag construct in a Cre-independent mannerDepositorInsertsynatophysin 9X HA tag T2A tdtomato
UseAAVTags9X HAExpressionMammalianPromotersynapsinAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pES.42.1c
Plasmid#69576PurposeTCR a chain cDNA in pES4 (transgenic construct) for creating OT-1 miceDepositorAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP
Plasmid#172001PurposeExpression for LIBRON constructsDepositorInsertFKBP DD (L106P)-UbK0G75C
UseRetroviralTagssfGFPMutationchange all lysin residues of ubiquitin to Arg, G7…Available SinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMF-TetTight
Plasmid#193288PurposeLentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgeneDepositorInsertrtTA-P2A-tTS
UseLentiviralExpressionMammalianPromoterEF‐1αAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330GFP-hU6-gRNA-hSpCas9
Plasmid#128385PurposeGFP positive gRNA/hSpCas9 constructDepositorInsertGFP, gRNA and hSpCas9
UseCRISPRAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
ExpressionMammalianAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH147
Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSyn-PreSynBar-hSyn-PreRNA short
Plasmid#247715PurposeSynBar protein and RNA construct used in HEK and neuron culture experiment.DepositorInsertPreSynBar and PreRNA short
UseAAVAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
hSyn-PostSynBar-hSyn-PostRNA short
Plasmid#247716PurposeSynBar protein and RNA construct used in HEK and neuron culture experiment.DepositorInsertPostSynBar and PostRNA short
UseAAVAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pADH119
Plasmid#90985PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C. albicans LEU2 2 of 2
UseCRISPRAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLUT1-GFP
Plasmid#89440PurposeGLUT1 constructDepositorAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
ExpressionMammalianMutationA206K bothAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pWM_12x601_20bpLinker
Plasmid#157787PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_20bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-mito-tagRFP
Plasmid#185599PurposeLentiviral construct for expression of tagRFP-T targeted to mitochondriaDepositorInsertmito-tagRFP-T
UseLentiviralExpressionMammalianAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
TagsSUMOExpressionBacterialAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_35bpLinker
Plasmid#157790PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_35bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM310-Myc
Plasmid#80053PurposeExpression of a Myc tagged version of the BlaM-Vpr fusion proteinDepositorInsertMyc-BlaM-Vpr
TagsMycExpressionMammalianMutationN-terminal Myc tag added to construct pMM310Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
hSyn-PreSynBar-hSyn-PreRNA barcode landing pad
Plasmid#247717PurposeThe set of SynBar constructs used in the optimized connectome-seq experimentsDepositorInsertPreSynBar and PreRNA
UseAAVAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-PostSynBar-P2A-mCherry
Plasmid#247719PurposeThe set of SynBar constructs used in the optimized connectome-seq experiments.DepositorInsertPostSynBar and mCherry
UseAAVAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
AB65_pCAG.Cas9-D10A.rBGpA
Plasmid#199256PurposeExpression construct encoding the SpCas9-D10A nickaseDepositorInsertSpCas9.D10A nickase
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
IQ-compete reporter (integrating)
Plasmid#247176PurposePlasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.DepositorInsertGFP-TCS-Quencher-CL1
ExpressionYeastPromoterTEF1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Atp5-DHFR-TEV (integrating)
Plasmid#247219PurposePlasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertAtp5 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Oxa1-DHFR-TEV (integrating)
Plasmid#247220PurposePlasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertOxa1 MTS-DHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-bReaches-TS
Plasmid#158701PurposeReporter constructDepositorInsertmCherry-p2A-bReaches-TS
UseAAVTagsmCherryExpressionMammalianPromoterTREAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico PGK GFP
Plasmid#12093PurposeCre-regulated lentiviral shRNA vector. Cre addition causes the EGFP gene to be recombined out of the construct, activating shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR2-EYFP
Plasmid#67877PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR2
UseLentiviralTagsEYFPExpressionMammalianPromoterUbCAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENExpressionMammalianPromoterCAG, EF-1 alpha, and TRE3GAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lyn-R-GECO1
Plasmid#120410PurposeExpresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.DepositorInsertLyn-R-GECO1
ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-mNFM
Plasmid#32909PurposeMammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein MDepositorAvailable SinceNov. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-hGtACR1-EYFP
Plasmid#67795PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR1
UseLentiviralTagsEYFPExpressionMammalianAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS MCK CRE
Plasmid#12529DepositorInsertCre recombinase
UseCre/Lox; Transgenic constructMutationCre is driven by the Muscle Creatine Kinase promo…Available SinceAug. 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCLAP
Plasmid#200786PurposeCloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.DepositorTypeEmpty backboneTagsCLIPf and SNAPf-6xHis-FLAGExpressionMammalianAvailable SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-SENP3-C526A
Plasmid#115522PurposeMammalian expression constructDepositorAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMF-FU_TetO_GW_PPARG2
Plasmid#193289PurposeLentiviral construct that expresses a dox-inducible human PPARG2DepositorAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only