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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

Plasmid

#198292

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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epB_CAG_TetRFlag-nls-GFPx2_DEx2

Plasmid

#119910

Purpose

ePiggyBac mammalian expression plasmid for TetR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-GFPx2

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Article

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; control_gRNA

Plasmid

#199336

Purpose

pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiHistoneNb

Plasmid

#198293

Purpose

Produces PVCs retargeted with A9 anti-histone nanobody

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiHistoneNb

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiALFAtagNb-TM

Plasmid

#198307

Purpose

Displays anti-ALFA nanobody from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiALFAtagNb-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-Halox2_DEx4

Plasmid

#119907

Purpose

ePiggyBac mammalian expression plasmid for CymR-Halo fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-Halox2

Expression

Mammalian

Available Since

April 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiMoonTagNb-TM

Plasmid

#198305

Purpose

Displays anti-MoonTag nanobody from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiMoonTagNb-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Expression

Bacterial

Promoter

Ec-TTL-P112 and BBa_J23116

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPAdGO2-PGK-Neo

Plasmid

#136899

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO2

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TAG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBS mPrx1 Cre (CT#644)

Plasmid

#13962

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 Cre (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG/HA-TNRC6B

Plasmid

#10979

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

TNRC6B (TNRC6B Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-FLAG

Plasmid

#198297

Purpose

Produces PVCs retargeted with FLAG tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-FLAG

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-TREX1

Plasmid

#164245

Purpose

retroviral plasmid for GFP-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-CD4DARPin

Plasmid

#198294

Purpose

Produces PVCs retargeted with anti-CD4 DARPin D27.2

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-CD4DARPin

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiEEscFv-TM

Plasmid

#198304

Purpose

Displays anti-EE scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiEEscFv-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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