We narrowed to 5,891 results for: dna plasmids

1. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRel-Hyg

   Plasmid

   #227582

   Purpose

   Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GT-CAG-mStayGold

   Plasmid

   #241387

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mStayGold
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-scFvGCN4sfGFPTET1CD

   Plasmid

   #82561

   Purpose

   Expression vector for antibody-sfGFP-TET1CD.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   scFvGCN4sfGFPTET1CD
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

   Plasmid

   #229791

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRNA-modified

   Plasmid

   #84477

   Purpose

   guide RNA expression lentiviral construct

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   guide RNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-FLAG-Ku70

   Plasmid

   #46957

   Purpose

   Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHelp

    Plasmid

    #227583

    Purpose

    Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    guide RNA scaffold
    

    Use

    Cloning vector

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. rs214-minor

    Plasmid

    #113380

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 minor allele
    

    Use

    Luciferase

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBP-SpyCatcher

    Plasmid

    #79053

    Purpose

    Expresses maltose binding protein (MBP) SpyCatcher fusion

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    MBP-SpyCatcher
    

    Tags

    6xHis and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Derived from pDEST14-SpyCatcher (Plasmid #35044)…

    Promoter

    T7

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmEos3.2:cat

    Plasmid

    #78100

    Purpose

    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

    Depositor

    Peng Chen

    Article

    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

    Insert

    mEos3.2-cat
    

    Expression

    Bacterial

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Bxb1-GT_CAG_3xTurboGFP-NLS

    Plasmid

    #229777

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AX53_pS.Donor.S1.TS

    Plasmid

    #100290

    Purpose

    AAVS1-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    18 bp RFLP (MluI+KspAI)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBT-peRNA_GG-Puro

    Plasmid

    #173220

    Purpose

    piggyBac transposon containing hU6 promotor and pegRNA cloning site

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    pegRNA cloning cassette
    PuroR
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1-a and hU6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKB01-PssbB-mKate2

    Plasmid

    #69105

    Purpose

    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mKate2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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