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pDONR223-STK11

Plasmid

#23802

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK11 (STK11 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pOpen-ETSSB

Plasmid

#165549

Purpose

ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Extreme Thermostable Single-Stranded DNA Binding Protein

Use

Synthetic Biology

Available since

Aug. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPR - EGFP sgRNA 5

Plasmid

#51764

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 5

Use

CRISPR and Lentiviral

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPos-Ma-CmR-TAG111

Plasmid

#197571

Purpose

Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

Depositor

Ryan Mehl

Publication

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

Chloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)

Expression

Bacterial

Mutation

8TAG/112TAG and 111TAG

Promoter

AraC, T7, cat promoter (constitutive), and lpp (c…

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Publication

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KEAP1 (KEAP1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTP434

Plasmid

#104167

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

3x Cysteines located at bps 493-495, 520-522, and…

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Publication

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RSV CREB

Plasmid

#22394

Depositor

Marc Montminy

Publication

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Publication

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMV306G13+FFlucRT

Plasmid

#49997

Purpose

Integrating vector with an improved Red-shifted Thermostable Firefly Luciferase

Depositor

Brian Robertson

Publication

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13 from M. marinum

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMM136

Plasmid

#127217

Purpose

BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, MP-delta, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mTagBFP2-Calnexin-N-14

Plasmid

#55278

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Calnexin (CANX Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCORE-UK

Plasmid

#72238

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
kanMX4

Expression

Bacterial

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCFPT7

Plasmid

#28012

Depositor

Lingchong You

Publication

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

T7 RNAP

Expression

Bacterial

Available since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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