We narrowed to 5,876 results for: nls

1. PIEZO1-HaloTag

   Plasmid

   #207834

   Purpose

   PIEZO1 fusion for biochemistry and live imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   PIEZO1 (PIEZO1 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniABEmax (pRZ900)

   Plasmid

   #131311

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-ABE7.10

   Plasmid

   #102919

   Purpose

   expresses ABE7.10 in mammalian cells

   Depositor

   David Liu

   Article

   Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

   Insert

   ABE7.10
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NG-ABE8e(V106W)

   Plasmid

   #198533

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. KRAB-dCas9

   Plasmid

   #112195

   Purpose

   KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   KRAB (ZNF10 Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-KRAB-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpRY-ABE8e(V106W)

   Plasmid

   #198553

   Purpose

   Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-ABE8e(V106W)

   Plasmid

   #198547

   Purpose

   Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMJ922

   Plasmid

   #78312

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-EGFP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NG-YE1-BE4max

    Plasmid

    #198535

    Purpose

    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-YE1-BE4max

    Plasmid

    #198549

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NRCH-ABE8e(V106W)

    Plasmid

    #198556

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. xCas9(3.7)-ABE(7.10)

    Plasmid

    #108382

    Purpose

    Mammalian expression vector for xCas9(3.7)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VRQR variant

    Plasmid

    #138562

    Purpose

    Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, T1337R

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-CMV-Puro-miniAAVR-FLAG

    Plasmid

    #166717

    Purpose

    Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    miniAAVR-FLAG
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Only expressing PKD1-3 of ectodomain

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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